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Carbohydr Polym. 2019 Apr 1;209:350-355. doi: 10.1016/j.carbpol.2019.01.039. Epub 2019 Jan 14.

In vitro and in silico inhibition properties of fucoidan against α-amylase and α-D-glucosidase with relevance to type 2 diabetes mellitus.

Author information

1
CAS in Marine Biology, Annamalai University, Parangipettai, 608502, Tamil Nadu, India. Electronic address: lakshmanasenthil@gmail.com.
2
CAS in Marine Biology, Annamalai University, Parangipettai, 608502, Tamil Nadu, India.
3
Drug Discovery Lab, Department of Chemistry, Annamalai University, Chidambaram, 608002, Tamil Nadu, India.

Abstract

This study investigated the in vitro and in silico inhibitory properties of fucoidan extracted from Turbinaria conoides against α-amylase and α-D-glucosidase. Extracted fucoidan contained 59 and 35% of fucose and sulphate and was characterized using 1H NMR. Dose dependent inhibition assays showed maximum of 85 and 72% of inhibition for α-amylase and α-D-glucosidase at 2.07 and 1.03 μM concentration of fucoidan. The IC50 value of fucoidan was found to be 1.07 and 0.68 μM against α-amylase and α-D-glucosidase. In silico studies (grid based docking) by Schrödinger software revealed that fucoidan as a potent inhibitor for both α-amylase and α-D-glucosidase based on number of interactions, hydrogen bond length and binding energy. Furthermore, fucoidan fulfilled the pharmacokinetic properties thus promising to develop fucoidan as drug for type 2 DM.

KEYWORDS:

Fucoidan; Turbinaria conoides; Type 2 DM; α-Amylase; α-D-Glucosidase

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