Format

Send to

Choose Destination
J Dairy Res. 2019 Feb;86(1):94-97. doi: 10.1017/S0022029919000116. Epub 2019 Feb 7.

Rapid detection of cow milk adulteration/contamination in goat milk by a lateral flow colloidal gold immunoassay strip.

Author information

1
Department of Transfusion Medicine,School of Laboratory Medicine and Biotechnology, Southern Medical University,Guangzhou,China.
2
Shenzhen Key Research Laboratory of Detection Technology R&D on Food Safety,Technical Centre for Food Inspection and Quarantine, Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen,China.
3
Nanjing Entry-exit Inspection and Quarantine Bureau,Nanjing,China.

Abstract

Current available methods to detect cow milk adulteration or accidental contamination of goat milk are both laborious and time consuming. The aim of this technical research communication was to develop a simple, rapid, specific and sensitive method for quantitative detection of cow milk in goat milk. A competitive lateral flow immunoassay (LFIA) strip was developed using a specific monoclonal antibody (mAb) labeled with colloidal gold nanoparticles (GNPs) for specifically binding to cow milk casein. The detection limit of this rapid detection was 0.07% of cow milk in goat milk, providing equal specificity and higher sensitivity when compared with a commercial enzyme-linked immunosorbent assay (ELISA). These result suggest that the established rapid GNPs-LFIA strip could be used for monitoring cow milk adulteration/contamination of goat milk.

KEYWORDS:

Colloidal gold nanoparticles; contamination or adulteration; cow milk; goat milk; lateral flow immunoassay strip

PMID:
30729896
DOI:
10.1017/S0022029919000116
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Cambridge University Press
Loading ...
Support Center