Two-stage thermophilic anaerobic co-digestion of cattle manure and corn stover was conducted to increase biomethane production. The first stage pre-digestion of corn stover was studied based on the following treatment variables: corn stover to liquid fraction of digestate (CS:LFD) ratio (1:7, 1:10, 1:13, 1:14), digestion temperature (55 °C, 60 °C) and digestion time (3, 7, 14 days). The reduction in lignin, cellulose and hemicellulose (LCH) was between 3.97% and 11.98%, which increased the biodegradability of corn stover. Corn stover pre-digested with a CS:LFD ratio of 1:10 at 55 °C for a period of 3 and 7 days was subjected to anaerobic co-digestion with cattle manure. The highest biomethane yield was observed on day 21 with a value of 357.41 mL/g volatile solids (VS) for untreated corn stover, 446.84 mL/g VS for corn stover pre-digested for 3 days and 518.58 mL/g VS for corn stover pre-digested for 7 days with LFD. The VS conversion efficiency for co-digestion of cattle manure with untreated corn stover, corn stover pre-digested for 3 days and 7 days was 42.8%, 43.3% and 51.8%, respectively, on day 21, which was higher than that (34.0%) of cattle manure only.
Keywords: Anaerobic digestion; biomethane; cattle manure; co-digestion; corn stover; liquid fraction of digestate; pre-digestion; thermophilic.