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Ann Hematol. 2019 May;98(5):1197-1207. doi: 10.1007/s00277-019-03628-8. Epub 2019 Feb 7.

Clinicopathological features of HCV-positive splenic diffuse large B cell lymphoma.

Author information

1
Department of Pathology, Kurume University, School of Medicine, Asahimachi 67, Kurume, Fukuoka, 830-0011, Japan.
2
Department of Hematology, Hokkaido University Faculty of Medicine, Sapporo, Japan.
3
Department of Pathology, Kurume University, School of Medicine, Asahimachi 67, Kurume, Fukuoka, 830-0011, Japan. miyoshi_hiroaki@med.kurume-u.ac.jp.
4
Department of Medicine and Biosystemic Science, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan.
5
Department of Hematology, Hamanomachi Hospital, Fukuoka, Japan.
6
Department of Hematology, Kushiro Rosai Hospital, Kushiro, Japan.
7
Department of Hematology, Kurume University, School of Medicine, Kurume, Japan.

Abstract

The hepatitis C virus (HCV) is a single-stranded RNA virus which is thought to be involved in the onset of B cell lymphoma. HCV-positive diffuse large B cell lymphoma (DLBCL) has been reported to clinically manifest in extranodal lesions (e.g., in the liver, spleen, and stomach). Here, we investigated HCV-positive and -negative primary splenic DLBCL (p-spDLBCL) and non-primary splenic DLBCL (ordinary DLBCL). Furthermore, to examine HCV lymphomagenesis, RNA in situ hybridization (ISH), RT-PCR (reverse-transcription polymerase chain reaction), and NS3 immunostaining of HCV viral nonstructural proteins were performed. HCV-positive p-spDLBCL patients presented fewer B symptoms (asymptomatic) and better performance status, with elevated presence of splenic macronodular lesions and more germinal center B cell (GCB) sub-group cases than HCV-negative p-spDLBCL patients. However, HCV-positive ordinary DLBCL patients were found to have more non-GCB sub-group cases than HCV-negative ordinary DLBCL patients. HCV-positive DLBCL patients showed 20.6% (7/34) NS3 positivity, 16.7% (1/6) HCV-RNA in situ positivity, and 22.2% (2/9) detection of HCV-RNA in tumor tissue by RT-PCR. Splenic samples were found to have a higher frequency of HCV detection than lymph node samples, thus suggesting that HCV may be closely related to lymphomagenesis, especially in splenic lymphoma.

KEYWORDS:

Hepatitis C virus; Malignant lymphoma; NS3; Primary splenic DLBCL; RNA in situ hybridization

PMID:
30729289
DOI:
10.1007/s00277-019-03628-8
[Indexed for MEDLINE]

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