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Arch Dis Child Fetal Neonatal Ed. 2019 Feb 6. pii: fetalneonatal-2018-316117. doi: 10.1136/archdischild-2018-316117. [Epub ahead of print]

New short-term heat inactivation method of cytomegalovirus (CMV) in breast milk: impact on CMV inactivation, CMV antibodies and enzyme activities.

Author information

1
Department of Neonatology, University Hospital of Tuebingen, Tuebingen, Germany.
2
University Hospital Jena, Jena, Germany.
3
Institute of Medical Virology and Epidemiology of Viral Diseases, University Hospital of Tuebingen, Tubingen, Germany.

Abstract

OBJECTIVES:

Breast milk (BM) is the primary source of cytomegalovirus (CMV) transmission to premature infants with potentially harmful consequences. We therefore wanted to evaluate temperature and duration of short-term BM pasteurisation with respect to CMV inactivation, effect on CMV-IgG antibodies and BM enzyme activities.

METHODS:

116 artificially CMV-spiked BM and 15 wild-type virus-infected samples were subjected for 5 s to different temperatures (55°C-72°C). CMV-IE-1 expression in fibroblast nuclei was assessed using the milk whey fraction in short-term microculture. BM lipase and alkaline phosphatase (AP) activities and CMV binding using CMV-recomLine immunoblotting and neutralising antibodies using epithelial target cells were analysed before and after heating.

RESULTS:

A minimum of 5 s above 60°C was necessary for CMV inactivation in both CMV-AD-169 spiked and wild-type infected BM. Lipase was very heat sensitive (activities of 54% at 55°C, 5% at 60°C and 2% at 65°C). AP showed activities of 77%, 88% and 10%, respectively. CMV-p150 IgG antibodies were mostly preserved at 62°C for 5 s.

CONCLUSION:

Our results show that short-term pasteurisation of BM at 62°C for 5 s might be efficient for CMV inactivation and reduces loss of enzyme activities, as well as CMV binding, and functional CMV antibodies.

KEYWORDS:

CMV; CMV neutralization; enzymes in breast milk; holder milk pasteurization; human milk CMV antibodies; short term heat treatment

Conflict of interest statement

Competing interests: JM and KH are holding a patent on a method for short-term pasteurisation of human milk (Internat. Pat. Application WO 00/74494).

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