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Nephron. 2019;142(1):17-25. doi: 10.1159/000496619. Epub 2019 Feb 6.

Dynamic Thiol/Disulphide Homeostasis in Children with Nephrotic Syndrome.

Author information

1
Department of Pediatric Nephrology, Saglik Bilimleri University, Dr. Sami Ulus Maternity and Child Health and Diseases Training and Research Hospital, Ankara, Turkey, fmeryemesra@yahoo.com.
2
Department of Pediatrics and Adolescent Medicine, Saglik Bilimleri University, Dr. Sami Ulus Maternity and Child Health and Diseases Training and Research Hospital, Ankara, Turkey.
3
Department of Biochemistry, Yıldırım Beyazıt University Medical School, Ankara, Turkey.
4
Department of Pediatric Nephrology, Saglik Bilimleri University, Dr. Sami Ulus Maternity and Child Health and Diseases Training and Research Hospital, Ankara, Turkey.

Abstract

BACKGROUND:

There is a growing body of evidence showing that there is an association between oxidative damage and kidney injury in patients with nephrotic syndrome (NS). Dynamic thiol/disulphide homeostasis (DTDH) is an important antioxidant system. The aim of this study was to determine if DTDH could be used as a biomarker of antioxidant status in pediatric NS patients using a novel automated method.

METHODS:

The study included 39 patients with NS and 40 healthy controls. The DTDH level was measured using a calorimetric and automated method developed by Erel and Neselioglu. In the NS group, DTDH was measured during first-attack episode, relapse, and remission.

RESULTS:

The native thiol, total thiol, and disulphide levels were significantly lower, the native thiol/total thiol ratio was significantly lower, and the disulphide/native thiol and disulphide/total thiol ratios were significantly higher in the NS patients during relapse and first-attack episode than during remission and in the controls. In addition, the native thiol and total thiol levels, and the native thiol/total thiol ratio were significantly lower, and the disulphide/native thiol and disulphide/total thiol ratios were significantly higher in the NS group during remission than those in the control group.

CONCLUSIONS:

The present findings show that DTDH shifted toward disulphide formation in the NS patients, even during remission. A clearer understanding of the mechanism of this disruption to homeostasis in NS patients might lead to the development of novel therapeutic strategies.

KEYWORDS:

Anti-oxidants; Childhood; Nephrotic syndrome; Oxidative stress; Thiol/disulphide homeostasis

PMID:
30726856
DOI:
10.1159/000496619

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