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J Fluoresc. 2019 Mar;29(2):425-434. doi: 10.1007/s10895-019-02351-x. Epub 2019 Feb 6.

A Highly Selective "Turn-on" Fluorescent Probe for Detection of Fe3+ in Cells.

Author information

1
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an, Shaanxi, 710127, People's Republic of China.
2
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an, Shaanxi, 710127, People's Republic of China. baiyinjuan@nwu.edu.cn.

Abstract

A new "turn-on" fluorescent probe Py based on rhodamine and piperonaldehyde was designed and synthesized for detecting Fe3+ in cells. The free probe Py was non-fluorescent. While only upon addition of Fe3+, the significant increase of the fluorescence and color were observed which could be visible directly by "naked-eye". The probe Py shows high selectivity and sensitivity for Fe3+ over other common metal ions in EtOH-H2O (3/2, v/v) mixed solution. The association constant and the detection limit were calculated to be 4.81 × 104 M-1 and 1.18 × 10-8 mol/L respectively. The introduction of piperonaldehyde unit could increase probe rigidity which could enhance its optical properties. Meanwhile, the binding mode between Py and Fe3+ was found to be a 1:1 complex formation. The density functional theory (DFT) calculations were performed which would further confirm the recognition mechanism between probe Py and Fe3+. In addition, the probe has been proved to be reversible for detecting Fe3+. Moreover, the probe Py was used to detect Fe3+ in cells successfully.

KEYWORDS:

Cell imaging; DFT calculations; Fe3 +; Fluorescent probe; Rhodamine

PMID:
30725356
DOI:
10.1007/s10895-019-02351-x

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