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Food Chem. 2019 Mar 1;275:638-643. doi: 10.1016/j.foodchem.2018.09.093. Epub 2018 Sep 15.

Quantitative analysis of Alaska pollock in seafood products by droplet digital PCR.

Author information

1
Biotechnology Research Division, National Institute of Fisheries Science, 216, Gijanghaean-ro, Gijang-eup, Gijang-gun, Busan 46083, Republic of Korea.
2
Food Safety and Processing Research Division, National Institute of Fisheries Science, 216, Gijanghaean-ro, Gijang-eup, Gijang-gun, Busan 46083, Republic of Korea.
3
Department of Microbiology, College of Natural Science, Pukyong National University, 45 Yongso-ro, Nam-gu, Busan 48513, Republic of Korea.
4
Biotechnology Research Division, National Institute of Fisheries Science, 216, Gijanghaean-ro, Gijang-eup, Gijang-gun, Busan 46083, Republic of Korea. Electronic address: genetics@korea.kr.

Abstract

A highly accurate quantitative method, based on the new technique, droplet digital PCR (ddPCR), was applied to determine the content of Alaska pollock (Gadus chalcogrammus) in seafood products. Using this method, we found a linear relationship among raw sample weight, DNA concentration and DNA copy number. We also established a formula to calculate the raw sample weight, based on the number of DNA copies. To confirm the accuracy and applicability of this method, mixed samples of known composition were analyzed. Results from this study indicated that the ddPCR method described is suitable for quantifying Alaska pollock in seafood products and has the potential applied to a variety of tasks in food quality certification.

KEYWORDS:

Alaska pollock; Droplet digital PCR; Quantitative analysis; Seafood products

PMID:
30724244
DOI:
10.1016/j.foodchem.2018.09.093
[Indexed for MEDLINE]

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