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Stem Cells. 2019 May;37(5):623-630. doi: 10.1002/stem.2986. Epub 2019 Feb 22.

Functional Equivalency in Human Somatic Cell Nuclear Transfer-Derived Endothelial Cells.

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Department of Stem Cell Biology, Konkuk University, School of Medicine, Seoul, Republic of Korea.
CHA Stem Cell Institute, CHA University, Seongnam, Republic of Korea.
Division of research, BYON Co. Ltd., Stem Cell Research Center, Seoul, Republic of Korea.
Department of R&D, Advanced Bio Micro (ABM) Scientific Co., Cheonan, Republic of Korea.
Research Institute for Stem Cell Research, CHA Health Systems, Los Angeles, California, USA.
Department of Biomedical Science, CHA University, Seongnam, Republic of Korea.
Department of Stem Cell Biology, Research Institute, T&R Biofab Co. Ltd., Siheung, Republic of Korea.


The derivation of human embryonic stem cells (hESCs) by somatic cell nuclear transfer (SCNT) has prompted a re-emerging interest in using such cells for therapeutic cloning. Despite recent advancements in derivation protocols, the functional potential of CHA-NT4 derived cells is yet to be elucidated. For this reason, this study sought to differentiate CHA-NT4 cells toward an endothelial lineage in order to evaluate in vitro and in vivo functionality. To initial differentiation, embryoid body formation of CHA-NT4 was mediated by concave microwell system which was optimized for hESC-endothelial cell (EC) differentiation. The isolated CD31+ cells exhibited hallmark endothelial characteristics in terms of morphology, tubule formation, and ac-LDL uptake. Furthermore, CHA-NT4-derived EC (human nuclear transfer [hNT]-ESC-EC) transplantation in hind limb ischemic mice rescued the hind limb and restored blood perfusion. These findings suggest that hNT-ESC-EC are functionally equivalent to hESC-ECs, warranting further study of CHA-NT4 derivatives in comparison to other well established pluripotent stem cell lines. This revival of human SCNT-ESC research may lead to interesting insights into cellular behavior in relation to donor profile, mitochondrial DNA, and oocyte quality. Stem Cells 2019;37:623-630.


Cell therapy; Cell transplantation; Endothelial cell; Human somatic cell nuclear transfer; Ischemia


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