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J Cell Biochem. 2019 Feb 5. doi: 10.1002/jcb.28411. [Epub ahead of print]

Enhanced expression of circ_0000267 in hepatocellular carcinoma indicates poor prognosis and facilitates cell progression by sponging miR-646.

Author information

1
Department of General Division, Hongqi Affiliated Hospital of Mudanjiang Medical University, Mudanjiang, China.
2
Department of Ultrasound, The First People's Hospital of Mudanjiang, Mudanjiang, China.
3
Department of Radiology, Hongqi Affiliated Hospital of Mudanjiang Medical University, Mudanjiang, China.
4
Department of Cardiac Intensive Care Unit, Hongqi Affiliated Hospital of Mudanjiang Medical University, Mudanjiang, China.
5
Department of Prevention Medicine, College of Public Health, Mudanjiang Medical University, Mudanjiang, China.

Abstract

Hepatocellular carcinoma (HCC) is a highly aggressive carcinoma worldwide. Circular RNAs (circRNAs) have been proved to be involved in the pathogenesis of several carcinomas. circ_0000267 was reported to be elevated in HCC tissue samples by circRNA microarray. In this study, quantitative reverse-transcription polymerase chain reaction was induced to further detect the expression of circ_0000267 in HCC tissues and cells. The clinical significance was also explored by Fisher's exact test, Kaplan-Meier curves and Cox regression analysis. Cell counting kit-8, colony formation, flow cytometry and transwell experiments were conducted on HCC cells to elucidate the functions of circ_0000267. Dual-luciferase reporter assay was induced to explore the mechanism of circ_0000267. Moreover, rescue experiments were also performed on HCC cells. As a result, circ_0000267 was enhanced in HCC tissues and cell lines. This upregulation is associated with patients' clinical severity and poor prognosis. Functionally, circ_0000267 could facilitate cell growth, migration and invasion and attenuate cell apoptosis in HCC cells. Mechanistically, circ_0000267 could directly sponge miR-646 to exert its oncogenic properties. In summary, we identified a novel HCC-associated circRNA in the progression of this fatal disease.

KEYWORDS:

circ_0000267; circular RNA; hepatocellular carcinoma; miR-646; prognosis

PMID:
30719761
DOI:
10.1002/jcb.28411

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