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J Cell Biochem. 2019 Feb 5. doi: 10.1002/jcb.28411. [Epub ahead of print]

Enhanced expression of circ_0000267 in hepatocellular carcinoma indicates poor prognosis and facilitates cell progression by sponging miR-646.

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Department of General Division, Hongqi Affiliated Hospital of Mudanjiang Medical University, Mudanjiang, China.
Department of Ultrasound, The First People's Hospital of Mudanjiang, Mudanjiang, China.
Department of Radiology, Hongqi Affiliated Hospital of Mudanjiang Medical University, Mudanjiang, China.
Department of Cardiac Intensive Care Unit, Hongqi Affiliated Hospital of Mudanjiang Medical University, Mudanjiang, China.
Department of Prevention Medicine, College of Public Health, Mudanjiang Medical University, Mudanjiang, China.


Hepatocellular carcinoma (HCC) is a highly aggressive carcinoma worldwide. Circular RNAs (circRNAs) have been proved to be involved in the pathogenesis of several carcinomas. circ_0000267 was reported to be elevated in HCC tissue samples by circRNA microarray. In this study, quantitative reverse-transcription polymerase chain reaction was induced to further detect the expression of circ_0000267 in HCC tissues and cells. The clinical significance was also explored by Fisher's exact test, Kaplan-Meier curves and Cox regression analysis. Cell counting kit-8, colony formation, flow cytometry and transwell experiments were conducted on HCC cells to elucidate the functions of circ_0000267. Dual-luciferase reporter assay was induced to explore the mechanism of circ_0000267. Moreover, rescue experiments were also performed on HCC cells. As a result, circ_0000267 was enhanced in HCC tissues and cell lines. This upregulation is associated with patients' clinical severity and poor prognosis. Functionally, circ_0000267 could facilitate cell growth, migration and invasion and attenuate cell apoptosis in HCC cells. Mechanistically, circ_0000267 could directly sponge miR-646 to exert its oncogenic properties. In summary, we identified a novel HCC-associated circRNA in the progression of this fatal disease.


circ_0000267; circular RNA; hepatocellular carcinoma; miR-646; prognosis


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