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Nat Biotechnol. 2019 Feb;37(2):139-143. doi: 10.1038/s41587-018-0007-9. Epub 2019 Feb 4.

Resistance gene cloning from a wild crop relative by sequence capture and association genetics.

Author information

1
John Innes Centre, Norwich Research Park, Norwich, UK.
2
Commonwealth Scientific and Industrial Research Organization Agriculture and Food, Canberra, Australian Capital Territory, Australia.
3
Department of Plant Sciences, North Dakota State University, Fargo, ND, USA.
4
Department of Plant Pathology, University of Minnesota, St. Paul, MN, USA.
5
Arbor Biosciences, Ann Arbor, MI, USA.
6
Wheat Genetics Resource Center, Department of Plant Pathology, Kansas State University, Manhattan, KS, USA.
7
Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia.
8
Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, Queensland, Australia.
9
US Department of Agriculture, Agriculture Research Service, Cereal Disease Laboratory, St. Paul, MN, USA.
10
The University of Sydney Plant Breeding Institute, Cobbitty, New South Wales, Australia.
11
The Sainsbury Laboratory, Norwich Research Park, Norwich, UK.
12
The John Bingham Laboratory, NIAB, Cambridge, UK.
13
Michigan State University, East Lansing, MI, USA.
14
US Department of Agriculture, Agriculture Research Service, Northern Crop Science Laboratory, Cereal Crops Research Unit, Red River Valley Agricultural Research Center, Fargo, ND, USA.
15
John Innes Centre, Norwich Research Park, Norwich, UK. brande.wulff@jic.ac.uk.

Abstract

Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq enables R gene cloning in any crop that has a diverse germplasm panel.

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PMID:
30718880
DOI:
10.1038/s41587-018-0007-9
[Indexed for MEDLINE]

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