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Biochem Biophys Res Commun. 2019 Mar 12;510(3):416-420. doi: 10.1016/j.bbrc.2019.01.116. Epub 2019 Feb 2.

Long non-coding RNA XIST regulates miR-106b-5p/P21 axis to suppress tumor progression in renal cell carcinoma.

Author information

1
Department of Urology, The First Affiliated Hospital, Zhengzhou University, No 1 Jianshe East Rd., Zhengzhou, 450052, People's Republic of China; Urological Institute of Henan, Zhengzhou, 450052, Henan Province, People's Republic of China.
2
Department of Urology, The First Affiliated Hospital, Zhengzhou University, No 1 Jianshe East Rd., Zhengzhou, 450052, People's Republic of China.
3
Department of Neurology, The First Affiliated Hospital, Zhengzhou University, No 1 Jianshe East Rd., Zhengzhou, 450052, People's Republic of China.
4
Department of Urology, The First Affiliated Hospital, Zhengzhou University, No 1 Jianshe East Rd., Zhengzhou, 450052, People's Republic of China; Urological Institute of Henan, Zhengzhou, 450052, Henan Province, People's Republic of China. Electronic address: yjjsk2018@163.com.

Abstract

Long non-coding RNAs (lncRNAs) have been demonstrated to exert important roles in cancer development and progression. The biological function of lncRNA X-inactive specific transcript (XIST) in the development of renal cell carcinoma (RCC) and the underlying mechanisms are still largely unknown. In this study, we found that XIST was down-regulated in RCC tissues and cells. Overexpression of XIST significantly suppressed cell proliferation and induced cell G0/G1 arrest in vitro and inhibited tumor growth in vivo. We further found that XIST could directly interact with miR-106b-5p and increase the expression of P21. Thus, XIST positively regulated the expression of P21 through sponging miR-106b-5p, and played a tumor suppressor role in RCC. Moreover, we found that curcumin could regulate XIST/miR-106b-5p/P21 axis in RCC cells. Our study exhibits the role of XIST as a miRNA sponge in RCC and supports the potential application of XIST in RCC therapy.

KEYWORDS:

Cell proliferation; Curcumin; P21; XIST; miR-106b-5p

PMID:
30717973
DOI:
10.1016/j.bbrc.2019.01.116

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