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Mol Gen Genet. 1988 Dec;215(1):146-51.

Killing of Escherichia coli cells modulated by components of the stability system ParD of plasmid R1.

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Centro de Investigaciones Biológicas, (C.S.I.C.), Madrid, Spain.


The proteins P10 and P12 have been shown to be gene products of a new stability system, ParD, of plasmid R1. It is now shown that an R1 miniplasmid, pAB112, carrying a trans-complementable amber mutation in the gene of the P10 protein, is lethal for the host in the absence of suppression. This lethal effect is suppressed in a supF background and also by deletions in pAB112 that affect the gene of the P12 protein. These data indicate that the P12 protein has a lethal effect on the host and that this effect is neutralized by the P10 protein. The possibility that the stabilization conferred by the ParD system could be due to a counterselection, mediated by P12, of cells that lose the plasmid at cell division, is discussed.

[Indexed for MEDLINE]

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