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Mol Gen Genet. 1988 Dec;215(1):1-9.

The use of the luxA gene of the bacterial luciferase operon as a reporter gene.

Author information

1
Department of Plant Physiology, University of UmeƄ, Sweden.

Abstract

Bacterial luciferase can be assayed rapidly and with high sensitivity both in vivo and in vitro. Here we demonstrate that the N-terminal hydrophobic domain of the alpha catalytic subunit of the luciferase enzyme is indispensable for enzyme activity, although N-terminal translational fusions with full luciferase activity can be obtained. Bacterial luciferase is therefore ideally suited as a reporter enzyme for gene fusion experiments. A list of vectors for the convenient use of the luciferase marker genes to monitor gene expression in vivo are presented.

PMID:
3071733
DOI:
10.1007/bf00331295
[Indexed for MEDLINE]

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