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Am J Physiol Gastrointest Liver Physiol. 2019 Apr 1;316(4):G482-G494. doi: 10.1152/ajpgi.00262.2018. Epub 2019 Feb 4.

Preservation of reserve intestinal epithelial stem cells following severe ischemic injury.

Author information

1
Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University , Raleigh, North Carolina.
2
Center for Gastrointestinal Biologyand Disease, Joint Center at University of North Carolina Chapel Hill and North Carolina State University , Raleigh,North Carolina.
3
Department of Molecular and Biological Sciences, College of Veterinary Medicine, North Carolina State University , Raleigh, North Carolina.
4
University of North Carolina , Chapel Hill, North Carolina.

Abstract

Intestinal ischemia is an abdominal emergency with a mortality rate >50%, leading to epithelial barrier loss and subsequent sepsis. Epithelial renewal and repair after injury depend on intestinal epithelial stem cells (ISC) that reside within the crypts of Lieberk├╝hn. Two ISC populations critical to epithelial repair have been described: 1) active ISC (aISC; highly proliferative; leucine-rich-repeat-containing G protein-coupled receptor 5 positive, sex determining region Y-box 9 positive) and 2) reserve ISC [rISC; less proliferative; homeodomain only protein X (Hopx)+]. Yorkshire crossbred pigs (8-10 wk old) were subjected to 1-4 h of ischemia and 1 h of reperfusion or recovery by reversible mesenteric vascular occlusion. This study was designed to evaluate whether ISC-expressing biomarkers of aISCs or rISCs show differential resistance to ischemic injury and different contributions to the subsequent repair and regenerative responses. Our data demonstrate that, following 3-4 h ischemic injury, aISC undergo apoptosis, whereas rISC are preserved. Furthermore, these rISC are retained ex vivo in spheroids in which cell populations are enriched in the rISC biomarker Hopx. These cells appear to go on to provide a proliferative pool of cells during the recovery period. Taken together, these data indicate that Hopx+ cells are resistant to injury and are the likely source of epithelial renewal following prolonged ischemic injury. It is therefore possible that targeting reserve stem cells will lead to new therapies for patients with severe intestinal injury. NEW & NOTEWORTHY The population of reserve less-proliferative intestinal epithelial stem cells appears resistant to injury despite severe epithelial cell loss, including that of the active stem cell population, which results from prolonged mesenteric ischemia. These cells can change to an activated state and are likely indispensable to regenerative processes. Reserve stem cell targeted therapies may improve treatment and outcome of patients with ischemic disease.

KEYWORDS:

intestine; ischemia reperfusion injury; large animal model; reserve stem cells; stem cell

PMID:
30714814
PMCID:
PMC6483022
[Available on 2020-04-01]
DOI:
10.1152/ajpgi.00262.2018
[Indexed for MEDLINE]

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