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Gut Microbes. 2019 Feb 3:1-11. doi: 10.1080/19490976.2018.1564431. [Epub ahead of print]

The intestinal microbiota predisposes to traveler's diarrhea and to the carriage of multidrug-resistant Enterobacteriaceae after traveling to tropical regions.

Author information

1
a Genomic Research Laboratory, Service des Maladies Infectieuses , Hôpitaux Universitaires de Genève , Genève , Suisse.
2
b AP-HP, Hôpital Bichat, Département d'Epidémiologie et Recherche Clinique , URC Paris-Nord , F-75018 Paris , France.
3
c INSERM, CIC 1425-EC , UMR1123 , F-75018 Paris, France.
4
d Université Paris Diderot , UMR 1123, Sorbonne Paris Cité , F-75018 Paris, France.
5
e AP-HP, Hôpital Bichat, Service des Maladies Infectieuses et Tropicales , F-75018 Paris, France.
6
f INSERM and Université Paris Diderot , UMR 1137 IAME , F-75018 Paris, France.
7
g Laboratoire de Bactériologie , AP-HP, Hôpital Bichat.
8
h AP-HP, Hôpital Bichat , Laboratoire de Bactèriologie , F-75018 Paris , France.

Abstract

The risk of acquisition of multidrug-resistant Enterobacteriaceae (MRE) and of occurrence of diarrhea is high when traveling to tropical regions. The relationships between these phenomena and the composition of human gut microbiota have not yet been assessed. Here, we investigated the dynamics of changes of metabolically active microbiota by sequencing total RNA from fecal samples taken before and after travel to tropical regions. We included 43 subjects who could provide fecal samples before and after a travel to tropical regions. When found positive by culturing for any MRE after travel, the subjects sent an additional sample 1 month later. In all, 104 fecal samples were considered (43 before travel, 43 at return, 18 one month after travel). We extracted the whole RNA, performed retrotranscription and sequenced the cDNA (MiSeq 2x300bp). The reads were mapped to the reference operational taxonomic units (OTUs) and species/strains using the 16S Greengenes and 23S SILVA databases. We found that the occurrence of diarrhea during the travel was associated with a higher relative abundance of Prevotella copri before departure and after return. The composition of microbiota, before travel as well as at return, was not correlated with the acquisition of MRE. However, the clearance of MRE one month after return was linked to a specific pattern of bacterial species that was also found before and after return. In conclusion, we found specific OTUs associated to a higher risk of diarrhea during a stay in tropical regions and to a faster clearance of MRE after their acquisition.

KEYWORDS:

Multidrug-resistant Enterobacteriaceae; gut microbiota; traveller’s diarrhea; travelling to tropical regions

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