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Med Gas Res. 2019 Jan 9;8(4):154-159. doi: 10.4103/2045-9912.248266. eCollection 2018 Oct-Dec.

Effects of hydrogen on polarization of macrophages and microglia in a stroke model.

Author information

1
Department of Navy Aeromedicine, Faculty of Naval Medicine, Navy Medical University, Shanghai, China.
2
Department of Diving and Hyperbaric Medicine, Faculty of Naval Medicine, Navy Medical University, Shanghai, China.

Abstract

It has been confirmed that inflammation plays an important role in the pathogenesis of ischemic stroke. The polarization of microglia as an important participant in the inflammation following stroke is also found to be involved in stroke. This study aimed to investigate the effects of hydrogen gas on the polarization of macrophages/microglia in vitro. Raw264.7 cells were treated with lipopolysaccharides and then exposed to hydrogen. The microglia were treated with the supernatant from oxygen and glucose deprivation-treated neurons and then exposed to hydrogen. The phenotypes of Raw 264.7 cells and microglia were determined by flow cytometry, and cell morphology was observed. Results showed lipopolysaccharides significantly increased the M1 macrophages, and the supernatant from oxygen and glucose deprivation-treated neurons dramatically elevated the proportion of M1 microglia, but both treatments had little influence on the M2 cells. In addition, hydrogen treatment significantly inhibited the increase in M1 cells, but had no influence on M2 ones. Our findings suggest that the neuroprotection of hydrogen may be related to its regulation of microglia in the nervous system after stroke.

KEYWORDS:

hydrogen; inflammation; macrophages; microglia; polarization; stroke

Conflict of interest statement

Conflicts of interest The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported.

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