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Vet Immunol Immunopathol. 2019 Feb;208:34-43. doi: 10.1016/j.vetimm.2018.11.018. Epub 2018 Dec 21.

Evaluation of a viral DNA-protein immunization strategy against African swine fever in domestic pigs.

Author information

1
CBMSO-CSIC-UAM, C/ Nicolás Cabrera 1, Campus de Cantoblanco, Madrid, 28049, Spain.
2
Department of Diagnostic Medicine & Pathobiology, College of Veterinary Medicine, Kansas State University, K218 Mosier hall, 1800 Denison Ave, Manhattan, KS, 66506, USA.
3
Department of Microbiology and Immunology, Arizona College of Osteopathic Medicine, Midwestern University, Glendale, AZ, 85308, USA.
4
Department of Diagnostic Medicine & Pathobiology, College of Veterinary Medicine, Kansas State University, K218 Mosier hall, 1800 Denison Ave, Manhattan, KS, 66506, USA. Electronic address: jricht@vet.k-state.edu.
5
CBMSO-CSIC-UAM, C/ Nicolás Cabrera 1, Campus de Cantoblanco, Madrid, 28049, Spain. Electronic address: yrevilla@cbm.csic.es.

Abstract

African swine fever virus (ASFV) causes serious disease in domestic pigs for which there is no vaccine currently available. ASFV is a large DNA virus that encodes for more than 150 proteins, thus making the identification of viral antigens that induce a protective immune response difficult. Based on the functional roles of several ASFV proteins found in previous studies, we selected combinations of ASFV recombinant proteins and pcDNAs-expressing ASFV genes, to analyze their ability to induce humoral and cellular immune responses in pigs. Pigs were immunized using a modified prime-boost approach with combinations of previously selected viral DNA and proteins, resulting in induction of antibodies and specific cell-mediated immune response, measured by IFN-γ ELISpots. The ability of antibodies from pigs immunized with various combinations of ASFV-specific antigens to neutralize infection in vitro, and antigen-specific activation of the cellular immune response were analyzed.

KEYWORDS:

African swine fever virus; Heterologous DNA-protein immunization; Immune response; Immunopathology; Plasmid-expressed antigen; Recombinant protein

PMID:
30712790
DOI:
10.1016/j.vetimm.2018.11.018
[Indexed for MEDLINE]
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