Format

Send to

Choose Destination
Comp Immunol Microbiol Infect Dis. 2019 Feb;62:7-12. doi: 10.1016/j.cimid.2018.11.008. Epub 2018 Nov 24.

Development and characterization of mouse monoclonal antibodies to eight human complement components: Analysis of reactivity with orthologs of nine mammalian genera.

Author information

1
Unidad de Inmunología Microbiana, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain; Experimental Hepatology and Drug Targeting (HEVEFARM), University of Salamanca, IBSAL, CIBERehd, Salamanca, Spain.
2
VISAVET Health Surveillance Centre, Universidad Complutense de Madrid, Madrid, Spain.
3
Unidad de Inmunología Microbiana, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain. Electronic address: imoreno@isciii.es.
4
Unidad de Inmunología Microbiana, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain.
5
Experimental Hepatology and Drug Targeting (HEVEFARM), University of Salamanca, IBSAL, CIBERehd, Salamanca, Spain.

Abstract

To study complement function in mammalian leishmanioses, we developed mouse monoclonal antibodies to the human complement system components C1q, C4, factor D, factor H, factor B, properdin, C5 and C9. Antibody specificity was determined by indirect and capture ELISA and by Western blot. In flow cytometry analysis, seven antibodies recognized the cognate component on human serum-opsonized Leishmania promastigotes. Antibody reactivity was screened against promastigotes opsonized with sera of nine mammalian genera: pig, guinea pig, goat, rabbit, cat, dog, hamster, jird and rat. No antibody recognized jird epitopes on promastigotes. Anti-C4, -properdin, and -C5b reacted with the orthologous protein of all other mammals tested except cat (anti-properdin) and hamster (anti-C5b); anti-C9 only recognized the rabbit ortholog, and anti-C1q, -factor B and -factor H did not react with any of the nine orthologs. Such interspecies crossreactive antibodies can be valuable tools for analysis of mammalian complement function in infectious diseases.

KEYWORDS:

Antibody specificity; Leishmania promastigote; Mammalian complement; Monoclonal antibodies

PMID:
30711050
DOI:
10.1016/j.cimid.2018.11.008
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center