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Biosens Bioelectron. 2019 Mar 15;129:175-181. doi: 10.1016/j.bios.2018.12.058. Epub 2019 Jan 30.

A flyover style microfluidic chip for highly purified magnetic cell separation.

Author information

1
School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, PR China; Shanghai Engineering Research Center for Intelligent Diagnosis and Treatment Instrument, Shanghai 200240, PR China; Key Lab. for Thin Film and Microfabrication Technology of Ministry of Education, Shanghai 200240, PR China.
2
School of Biomedical Engineering, Institute for Personalized Medicine, Shanghai Jiao Tong University, Shanghai 200030, PR China.
3
School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, PR China; Shanghai Engineering Research Center for Intelligent Diagnosis and Treatment Instrument, Shanghai 200240, PR China; Key Lab. for Thin Film and Microfabrication Technology of Ministry of Education, Shanghai 200240, PR China. Electronic address: dchen@sjtu.edu.cn.
4
School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, PR China.
5
School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore 639798, Singapore.

Abstract

White blood cells (WBCs) isolated from peripheral blood have been verified as important biomarkers for the diagnosis, treatment and prognosis of cancer. However, it's still under challenge to acquire high-purity WBCs, even by taking advantage of current microfluidic technology. Considering the universality of clinical magnetic activated cell sorting (MACS) method, new developments on microfluidic chip in combination of magnetic cells separation technologies may provide a fascinating approach for high-purity WBCs sorting and widely clinical application. Here, we present a flyover style microfluidic chip which has been elaborately embedded with two-stage magnetic separation in continuous flow for WBCs sorting. Immunomagnetic micro/nano-particles (IMNPs) labeled WBC (WBC@IMNPs) were sequentially separated by a lateral magnetic force and a vertical magnetic force, and the final separation purity of WBCs reached up to 93 ± 1.67% at a flow rate of 20 μL min-1. Furthermore, the WBCs viability was up to 97.5 ± 1.8%. Consequently, this novel flyover style microfluidic-chip with magnetic separation technology has been successfully demonstrated as cut-in-edge method for high-purity WBCs sorting, and obviously it's easy to extend for other types of cells sorting under great potential application in biomedical fields.

KEYWORDS:

Cell sorting; Continuous flow; Magnetic separation; Microfluidic chip

PMID:
30710755
DOI:
10.1016/j.bios.2018.12.058
[Indexed for MEDLINE]

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