Discovery and characterization of a novel C-terminal peptide carboxyl methyltransferase in a lassomycin-like lasso peptide biosynthetic pathway

Appl Microbiol Biotechnol. 2019 Mar;103(6):2649-2664. doi: 10.1007/s00253-019-09645-x. Epub 2019 Feb 1.

Abstract

Lasso peptides belong to a peculiar family of ribosomally synthesized and post-translationally modified peptides (RiPPs)-natural products with an unusual isopeptide-bonded slipknot structure. Except for assembling of this unusual lasso fold, several further post-translational modifications of lasso peptides, including C-terminal methylation, phosphorylation/poly-phosphorylation, citrullination, and acetylation, have been reported recently. However, most of their biosynthetic logic have not been elucidated except the phosphorylated paeninodin lasso peptide. Herein, we identified two novel lassomycin-like lasso peptide biosynthetic pathways and, for the first time, characterized a novel C-terminal peptide carboxyl methyltransferase involved in these pathways. Our investigations revealed that this new family of methyltransferase could specifically methylate the C terminus of precursor peptide substrates, eventually leading to lassomycin-like C-terminal methylated lasso peptides. Our studies offer another rare insight into the extraordinary strategies of chemical diversification adopted by lasso peptide biosynthetic machinery and predicated two valuable sources for methylated lasso peptide discovery.

Keywords: Lasso peptide; Lassomycin; Methyltransferase; Ribosomally synthesized and post-translationally modified peptides.

MeSH terms

  • Actinobacteria / enzymology*
  • Bacterial Proteins / isolation & purification
  • Bacterial Proteins / metabolism*
  • Biological Products
  • Biosynthetic Pathways
  • Carboxyl and Carbamoyl Transferases / isolation & purification
  • Carboxyl and Carbamoyl Transferases / metabolism*
  • Methylation
  • Methyltransferases / isolation & purification
  • Methyltransferases / metabolism*
  • Peptide Biosynthesis
  • Peptides / metabolism*
  • Peptides, Cyclic
  • Phosphorylation
  • Protein Processing, Post-Translational
  • Ribosomes / metabolism
  • Streptomyces / enzymology*

Substances

  • Bacterial Proteins
  • Biological Products
  • Peptides
  • Peptides, Cyclic
  • lassomycin
  • Methyltransferases
  • Carboxyl and Carbamoyl Transferases