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Indian J Pathol Microbiol. 2019 Jan-Mar;62(1):54-60. doi: 10.4103/IJPM.IJPM_82_18.

Differential expression of cyclin E, p63, and Ki-67 in gestational trophoblastic disease and its role in diagnosis and management: A prospective case-control study.

Author information

Department of Pathology, S.C.B. Medical Colllege, Cuttack, Odisha, India.
Department of Obstetrics and Gynaecology, S.C.B. Medical Colllege, Cuttack, Odisha, India.
Department of Pathology, AHRCC, Cuttack, Odisha, India.



Gestational trophoblastic disease (GTD) constitutes a spectrum of tumors and tumor-like conditions, characterized by proliferation of pregnancy-associated trophoblastic tissue of progressive malignant potential. It is very difficult to differentiate these complex groups of lesions basing on histomorphology alone. Immunohistochemistry (IHC) with cyclin E, P63, and Ki-67 has a definite role in the identification of different trophoblasts and entities of GTD and also in the determination of biological behavior.


The aim of this study is to find the differential expression of cyclin E, p63, and Ki-67 in normal placenta, hydropic abortus (HA), and various entities of GTD.

Design and Settings:

A prospective case-control study conducted in a government medical college.


Total 96 cases, divided into Group A (48 histologically confirmed cases of GTD) and Group B (controls comprising 8 HA and 40 normal placentas of different trimesters), were studied. The histological samples were subjected to IHC using cyclin E, Ki-67, and p63.

Statistical Analysis:

Results were analyzed using SPSS statistical method.


Among the three immunomarkers used, Cyclin E and Ki-67 show statistically significant difference (P < 0.05) when compared between GTD and control groups, but it was insignificant for p63 (P = 0.369). Strong staining intensity of cyclin E and Ki-67 is seen in complete moles, choriocarcinoma, and placental site trophoblastic tumor.


This study was done to evaluate the role of cell cycle regulatory proteins such as cyclin E and p63 and proliferation marker Ki-67 in the detection of various trophoblasts and differential diagnosis of the lesions associated with them.


Complete hydatidiform mole; Cyclin E; Ki-67; gestational trophoblastic disease; immunohistochemistry; p63; partial hydatidiform mole; placental site trophoblastic tumor

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