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Indian J Pathol Microbiol. 2019 Jan-Mar;62(1):54-60. doi: 10.4103/IJPM.IJPM_82_18.

Differential expression of cyclin E, p63, and Ki-67 in gestational trophoblastic disease and its role in diagnosis and management: A prospective case-control study.

Author information

1
Department of Pathology, S.C.B. Medical Colllege, Cuttack, Odisha, India.
2
Department of Obstetrics and Gynaecology, S.C.B. Medical Colllege, Cuttack, Odisha, India.
3
Department of Pathology, AHRCC, Cuttack, Odisha, India.

Abstract

Background:

Gestational trophoblastic disease (GTD) constitutes a spectrum of tumors and tumor-like conditions, characterized by proliferation of pregnancy-associated trophoblastic tissue of progressive malignant potential. It is very difficult to differentiate these complex groups of lesions basing on histomorphology alone. Immunohistochemistry (IHC) with cyclin E, P63, and Ki-67 has a definite role in the identification of different trophoblasts and entities of GTD and also in the determination of biological behavior.

Aims:

The aim of this study is to find the differential expression of cyclin E, p63, and Ki-67 in normal placenta, hydropic abortus (HA), and various entities of GTD.

Design and Settings:

A prospective case-control study conducted in a government medical college.

Methods:

Total 96 cases, divided into Group A (48 histologically confirmed cases of GTD) and Group B (controls comprising 8 HA and 40 normal placentas of different trimesters), were studied. The histological samples were subjected to IHC using cyclin E, Ki-67, and p63.

Statistical Analysis:

Results were analyzed using SPSS statistical method.

Results:

Among the three immunomarkers used, Cyclin E and Ki-67 show statistically significant difference (P < 0.05) when compared between GTD and control groups, but it was insignificant for p63 (P = 0.369). Strong staining intensity of cyclin E and Ki-67 is seen in complete moles, choriocarcinoma, and placental site trophoblastic tumor.

Conclusion:

This study was done to evaluate the role of cell cycle regulatory proteins such as cyclin E and p63 and proliferation marker Ki-67 in the detection of various trophoblasts and differential diagnosis of the lesions associated with them.

KEYWORDS:

Complete hydatidiform mole; Cyclin E; Ki-67; gestational trophoblastic disease; immunohistochemistry; p63; partial hydatidiform mole; placental site trophoblastic tumor

PMID:
30706860
DOI:
10.4103/IJPM.IJPM_82_18
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