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J Ind Microbiol Biotechnol. 2019 May;46(5):675-685. doi: 10.1007/s10295-019-02141-1. Epub 2019 Jan 31.

Biological conversion of propane to 2-propanol using group I and II methanotrophs as biocatalysts.

Author information

1
Department of Chemical Engineering, Kyung Hee University, Yongin, Gyeonggi, 17104, Republic of Korea.
2
Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, 04107, Republic of Korea. narosu@sogang.ac.kr.
3
Department of Chemical Engineering, Kyung Hee University, Yongin, Gyeonggi, 17104, Republic of Korea. eunylee@khu.ac.kr.

Abstract

Propane is the main component of liquefied petroleum gas and is derived from crude oil processing. Methanotrophic bacteria can convert various alkanes using methane monooxygenase enzyme to primary alcohols. These are further oxidized to various aldehydes by alcohol dehydrogenases or methanol dehydrogenases. In this study, 2-propanol was produced from propane using the whole cells of Methylosinus trichosporium OB3b, Methylomicrobium alcaliphilum 20Z, and Methylomonas sp. DH-1 as the biocatalysts. The biocatalytic process of converting propane to 2-propanol was optimized by the use of several inhibitors and additives, such as EDTA, sodium phosphate, and sodium formate to prevent oxidation of 2-propanol to acetone and to enhance conversion of propane to propanol. The maximum titer of 2-propanol was 0.424 g/L, 0.311 g/L, and 0.610 g/L for Methylomonas sp. DH-1, M. alcaliphilum 20Z, and M. trichosporium OB3b whole cells, respectively. These results showed that type I and type II methanotrophs could be used as the potent biocatalyst for conversion of propane to propanol.

KEYWORDS:

2-Propanol; M. trichosporium OB3b; Methane monooxygenase; Methylomonas sp. DH-1; Propane

PMID:
30706246
DOI:
10.1007/s10295-019-02141-1

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