Format

Send to

Choose Destination
Chem Commun (Camb). 2019 Feb 14;55(15):2218-2221. doi: 10.1039/c8cc09634a.

A semisynthetic fluorescent protein assembly-based FRET probe for real-time profiling of cell membrane protease functions in situ.

Author information

1
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, Hunan University, Changsha, 410082, P. R. China. huangyan.hnu@gmail.com niezhou.hnu@gmail.com.

Abstract

A semisynthetic fluorescent protein assembly-based FRET probe (sFPAP) was proposed for cell membrane protease function assay. Here, the probe was anchored on a living cell membrane through a membrane inserting peptide (MIP) and was successfully utilized for in situ imaging of furin activity on the living cell membrane in real time.

PMID:
30706069
DOI:
10.1039/c8cc09634a
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Royal Society of Chemistry
Loading ...
Support Center