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Sci Rep. 2019 Jan 31;9(1):1086. doi: 10.1038/s41598-018-37763-5.

Leukocyte expression profiles reveal gene sets with prognostic value for seizure-free outcome following stereotactic laser amygdalohippocampotomy.

Author information

1
Center for Applied Genetics and Genomic Medicine, University of Arizona, Tucson, AZ, 85721, USA.
2
Arizona Research Labs Division of Biotechnology, University of Arizona, Tucson, AZ, 85721, USA.
3
Division of Neurosurgery, Department of Surgery, University of Arizona College of Medicine, Tucson, AZ, 85724, USA.
4
Department of Surgery, University of Arizona College of Medicine, Tucson, AZ, 85724, USA.
5
University of North Texas Health Science Center, Fort Worth, TX, 76107, USA.
6
Department of Neurology, University of Arizona College of Medicine, Tucson, AZ, 85724, USA.
7
Center for Applied Genetics and Genomic Medicine, University of Arizona, Tucson, AZ, 85721, USA. mfh@email.arizona.edu.
8
Department of Neurology, University of Arizona College of Medicine, Tucson, AZ, 85724, USA. mfh@email.arizona.edu.

Abstract

Among patients with intractable epilepsy, the most commonly performed surgical procedure is craniotomy for amygdalohippocampectomy (AH). Stereotactic laser amygdalohippocampotomy (SLAH) has also been recently employed as a minimally invasive treatment for intractable temporal lobe epilepsy (TLE). Among patients treated with AH and SLAH approximately 65% and 54% of patients become seizure-free, respectively. Therefore, selection criteria for surgical candidates with improved prognostic value for post-operative seizure-free outcome are greatly needed. In this study, we perform RNA sequencing (RNA-Seq) on whole blood leukocyte samples taken from 16 patients with intractable TLE prior to SLAH to test the hypothesis that pre-operative leukocyte RNA expression profiles are prognostic for post-operative seizure outcome. Multidimensional scaling analysis of the RNA expression data indicated separate clustering of patients with seizure free (SF) and non-seizure-free (NSF) outcomes. Differential expression (DE) analysis performed on SF versus NSF groups revealed 24 significantly differentially expressed genes (≥2.0-fold change, p-value < 0.05, FDR <0.05). Network and pathway analyses identified differential activation of pathways involved in lipid metabolism, morphology of oligodendrocytes, inflammatory response, and development of astrocytes. These results suggest that pre-operative leukocyte expression profiles have prognostic value for seizure outcome following SLAH.

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