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Biosci Biotechnol Biochem. 2019 Jan 31:1-5. doi: 10.1080/09168451.2019.1574204. [Epub ahead of print]

Monitoring ADP/ATP ratio in yeast cells using the fluorescent-protein reporter PercevalHR.

Author information

1
a Graduate School of Science and Technology , Nara Institute of Science and Technology , Nara , Japan.

Abstract

PercevalHR (Perceval High Resolution) is an artificially designed fluorescent protein, which changes its excitation spectrum based on the ADP/ATP ratio of the environment. Here we demonstrated that PercevalHR can be used for monitoring energy status of Saccharomyces cerevisiae cells, which are affected by diauxic shift and mitochondria inhibition, in a non-invasive and non-destructive manner.

KEYWORDS:

Fluorescent reporter: ATP: ADP; diauxic shift; yeast

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