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Mol Cells. 2019 Feb 28;42(2):175-182. doi: 10.14348/molcells.2018.0288. Epub 2019 Jan 24.

The MicroRNA-551a/MEF2C Axis Regulates the Survival and Sphere Formation of Cancer Cells in Response to 5-Fluorouracil.

Author information

1
Department of Biochemistry, The Catholic University of Korea College of Medicine, Seoul, Korea.
2
Department of Molecular Science and Technology, Ajou University, Suwon, Korea.

Abstract

microRNAs regulate a diverse spectrum of cancer biology, including tumorigenesis, metastasis, stemness, and drug resistance. To investigate miRNA-mediated regulation of drug resistance, we characterized the resistant cell lines to 5-fluorouracil by inducing stable expression of miRNAs using lenti-miRNA library. Here, we demonstrate miR-551a as a novel factor regulating cell survival after 5-FU treatment. miR-551a-expressing cells (Hep3B-lenti-miR-551a) were resistant to 5-FU-induced cell death, and after 5-FU treatment, and showed significant increases in cell viability, cell survival, and sphere formation. It was further shown that myocyte-specific factor 2C is the direct target of miR-551a. Our results suggest that miR-551a plays a novel function in regulating 5-FU-induced cell death, and targeting miR-551a might be helpful to sensitize cells to anti-cancer drugs.

KEYWORDS:

anti-cancer drug resistance; cell survival; hepatocellular carcinoma; microRNA; sphere formation

PMID:
30703870
PMCID:
PMC6399004
DOI:
10.14348/molcells.2018.0288
[Indexed for MEDLINE]
Free PMC Article

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