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Mol Gen Genet. 1988 Oct;214(2):263-70.

Yeast nuclear gene CBS2, required for translational activation of cytochrome b, encodes a basic protein of 45 kDa.

Author information

1
Institut für Genetik und Mikrobiologie der Universität München, Federal Republic of Germany.

Abstract

In yeast, synthesis of apocytochrome b from mitochondrial COB mRNA depends on at least three nuclear gene products. The translation stimulatory effect by two of these nuclear genes, CBS1 and CBS2, is mediated by the 5'-untranslated leader of COB mRNA. In this report, we show that CBS2 is located on chromosome IV and provide genetic evidence that the CBS2 gene encodes a polypeptide. Determination of the DNA sequence reveals a contiguous open reading frame of 1167 bp. The deduced polypeptide has a calculated molecular weight of 44.5 kDa and is characterized by a high content of positively charged amino acids. It has no significant homology to any known protein. The CBS2 gene is transcribed into low abundance mRNA species with a major transcription initiation site located 97 bp upstream from the ATG start codon next to a poly(dA-dT) stretch.

PMID:
3070350
[Indexed for MEDLINE]

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