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Int J Biol Macromol. 2019 May 1;128:435-443. doi: 10.1016/j.ijbiomac.2019.01.158. Epub 2019 Jan 29.

Structural elucidation of a glucan from Crataegus pinnatifida and its bioactivity on intestinal bacteria strains.

Author information

1
School of Pharmacy, Zunyi Medical University, 201 Dalian Road, Zunyi 563003, PR China; Glycochemistry and Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China; University of Chinese Academy of Science, No. 19A Yuquan Road, Beijing 100049, PR China.
2
Glycochemistry and Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China; University of Chinese Academy of Science, No. 19A Yuquan Road, Beijing 100049, PR China; School of Life Sciences, Shanghai University, No. 99, Shangda Road, Shanghai 200444, PR China.
3
Glycochemistry and Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China; University of Chinese Academy of Science, No. 19A Yuquan Road, Beijing 100049, PR China.
4
Glycochemistry and Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China; University of Chinese Academy of Science, No. 19A Yuquan Road, Beijing 100049, PR China. Electronic address: xiachen@simm.ac.cn.
5
School of Pharmacy, Zunyi Medical University, 201 Dalian Road, Zunyi 563003, PR China; Glycochemistry and Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China; University of Chinese Academy of Science, No. 19A Yuquan Road, Beijing 100049, PR China. Electronic address: dingkan@simm.ac.cn.

Abstract

Crataegus pinnatifida is widely used as functional food and traditional medicine in China for various applications. Polysaccharides may contribute to health benefits, such as modulating human gut microbiota (HGM). To investigate structure features of polysaccharides from this plant, fruits of C. pinnatifida were extracted with boiling water, yielding crude polysaccharides HAW. A novel polysaccharide, named HAW1-1, was pooled by DEAE Sepharose™ Fast Flow column and further purified by Sephacryl S-100 HR column. The molecular weight of HAW1-1 was 42.35 kDa. The carbohydrate content of HAW1-1 was 99.42% and no protein was detected. The monosaccharide analysis showed this polysaccharide was only composed of glucose. According to the partial acid hydrolysis, methylation analysis and NMR spectra, the backbone of HAW1-1 was consisted of 1,4-linked α-D-glucan and 1,4,6-linked α-D-glucan. The side chains were composed of 1,6-linked α-D-glucan and terminal-linked α-D-glucan. The bioactivity tests showed that HAW1-1 could promote the growth of three species of intestinal Bacteroides and produce short-chain fatty acids (SCFAs). The results suggested that HAW1-1 might be a potential prebiotic candidate to maintain intestinal homeostasis and improve human gut health.

KEYWORDS:

Bacteroides; Crataegus pinnatifida; Glucan; Human gut microbiota

PMID:
30703422
DOI:
10.1016/j.ijbiomac.2019.01.158
[Indexed for MEDLINE]

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