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Biosci Trends. 2019 Mar 14;13(1):86-90. doi: 10.5582/bst.2019.01008. Epub 2019 Jan 30.

Structural analyses of a hemolytic compound found in an extract of Hypsizygus marmoreus fruiting bodies at a low pH.

Author information

1
Technology Joint Management Office, Research Promotion Division, Tokai University.
2
Department of Applied Biochemistry, Tokai University.

Abstract

The current study determined the structure of a hemolytic compound found in an extract from the fruiting bodies of the edible mushroom Hypsizygus marmoreus when its pH was lowered. The hemolytic compound was purified using the modified Bligh and Dyer method followed by chromatography using reversed phase and silica gel columns. Structural analyses of the purified hemolytic compound were performed using NMR and ESI-MS. The deduced structure indicated a trans,trans-5,8-docosadienoic acid calcium salt. Although numerous proteinous hemolysins from various mushrooms have been described, the current study is the first to report on a low-molecular-weight hemolytic compound derived from an H. marmoreus extract.

KEYWORDS:

Docosadienoic acid; Hypsizygus marmoreus; hemolysis; mushroom

PMID:
30700653
DOI:
10.5582/bst.2019.01008
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