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BMC Genomics. 2019 Jan 30;20(1):97. doi: 10.1186/s12864-019-5458-y.

Comparison of splenocyte microRNA expression profiles of pigs during acute and chronic toxoplasmosis.

Hou Z1,2,3,4, Liu D1,2,3,4, Su S1,2,3,4, Wang L1,2,3,4, Zhao Z1,2,3,4, Ma Y1,2,3,4, Li Q1,2,3,4, Jia C1,2,3,4, Xu J1,2,3,4, Zhou Y5, Tao J6,7,8,9.

Author information

1
College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, People's Republic of China.
2
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou, 225009, People's Republic of China.
3
Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agri-food Safety and Quality, Ministry of Agriculture of China, Yangzhou, 225009, People's Republic of China.
4
Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou University, Yangzhou, 225009, People's Republic of China.
5
Jiangsu Institute of Parasitic Diseases, Wuxi, 214064, People's Republic of China.
6
College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, People's Republic of China. yzjptao@126.com.
7
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou, 225009, People's Republic of China. yzjptao@126.com.
8
Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agri-food Safety and Quality, Ministry of Agriculture of China, Yangzhou, 225009, People's Republic of China. yzjptao@126.com.
9
Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou University, Yangzhou, 225009, People's Republic of China. yzjptao@126.com.

Abstract

BACKGROUND:

Toxoplasma gondii is an obligate intracellular parasite that infects humans and other warm-blooded animals. Previous quantitative proteomic analyses of infected host cells revealed that the expression of many host proteins is modulated by T. gondii infection. However, at present limited data are available on the differentially expressed miRNAs (DEMs) associated with the pathology and host immune responses induced by acute and chronic infection with T. gondii in pigs in vivo. In this study, high-throughput sequencing was used to investigate expression profiles of spleen miRNAs at 10, 25 and 50 days post-infection (DPI) in pigs infected with Chinese I genotype strain T. gondii isolated from a dead pig.

RESULTS:

When compared to the control group, 34, 6 and 86 DEMs were found in spleens of infected pigs at 10, 25 and 50 DPI, respectively. Gene Ontology (GO) enrichment analysis of the target genes of DEMs showed that no GO terms were enriched at 25 DPI, whereas 28 and 241 GO terms, of which two and 215 were sample-specific, were significantly enriched at 10 and 50 DPI, respectively. The top 20 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of the target genes of DEMs included signal transduction, immune system, metabolism and diseases. miRNA-gene network analysis revealed that the DEMs played important roles in the host immune response to T. gondii infection by modulating expression levels of cellular immunity-related cytokines and immune-related C-type lectins.

CONCLUSION:

Our results not only showed that host miRNA expression is altered by T. gondii but also revealed differences in the regulation of key biological processes and pathways involved in host responses to acute versus chronic T. gondii infection. This will aid future research into miRNA-target interactions during T. gondii infection in pigs and the development of novel therapies against T. gondii.

KEYWORDS:

Acute and chronic infection; Host regulation; Pig; Spleen; Toxoplasma gondii; microRNA

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