Format

Send to

Choose Destination
Epigenomics. 2019 Apr;11(5):527-542. doi: 10.2217/epi-2018-0189. Epub 2019 Jan 31.

Comprehensive and combined omics analysis reveals factors of ischemia-reperfusion injury in liver transplantation.

Huang S1,2,3, Ju W1,2,3, Zhu Z1,2,3, Han M1,2,3, Sun C1,2,3, Tang Y1,2,3, Hou Y1,2,3, Zhang Z1,2,3, Yang J1,2,3, Zhang Y1,2,3, Wang L1,2,3, Lin F1,2,3, Chen H1,2,3, Xie R1,2,3, Zhu C1,2,3, Wang D1,2,3, Wu L1,2,3, Zhao Q1,2,3, Chen M1,2,3, Zhou Q4,5, Guo Z1,2,3, He X1,2,3.

Author information

1
Organ Transplant Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, PR China.
2
Guangdong Provincial Key Laboratory of Organ Donation & Transplant Immunology, Guangzhou 510080, PR China.
3
Guangdong Provincial International Cooperation Base of Science & Technology (Organ Transplantation), Guangzhou 510080, PR China.
4
Department of General Surgery, Hui Ya Hospital of The First Affiliated Hospital, Sun Yat-sen University, Huizhou, Guangdong 516081, PR China.
5
Department of Liver Surgery, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, PR China.

Abstract

AIM:

To explore molecular mechanisms underlying liver ischemia-reperfusion injury (IRI).

MATERIALS & METHODS:

Four Gene Expression Omnibus datasets comprising liver transplantation data were collected for a comprehensive analysis. A proteomic analysis was performed and used for correlations analysis with transcriptomic.

RESULTS & CONCLUSION:

Ten differentially expressed genes were co-upregulated in four Gene Expression Omnibus datasets, including ATF3, CCL4, DNAJB1, DUSP5, JUND, KLF6, NFKBIA, PLAUR, PPP1R15A and TNFAIP3. The combined analysis demonstrated ten coregulated genes/proteins, including HBB, HBG2, CA1, SLC4A1, PLIN2, JUNB, HBA1, MMP9, SLC2A1 and PADI4. The coregulated differentially expressed genes and coregulated genes/proteins formed a tight interaction network and could serve as the core factors underlying IRI. Comprehensive and combined omics analyses revealed key factors underlying liver IRI, and thus having potential clinical significance.

KEYWORDS:

comprehensive analysis; ischemia-reperfusion injury; liver transplantation; proteome; transcriptome;  combined analysis

PMID:
30700158
DOI:
10.2217/epi-2018-0189
Free full text

Supplemental Content

Full text links

Icon for Atypon
Loading ...
Support Center