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Gene. 1988 Sep 30;69(2):331-5.

Genetic characterization of the gene hupA encoding the HU-2 protein of Escherichia coli.

Author information

1
Laboratory of Molecular Genetics, Tsukuba Life Science Center, Institute of Physical and Chemical Research, Ibaraki, Japan.

Abstract

The gene hupA encoding the HU-2 (HU-alpha) protein of Escherichia coli was mapped between rpoBC at 90 min and metA at 90.5 min on the K-12 genome by plasmid integration and P1-mediated transduction studies. Genetic studies using plasmid rescue techniques and physical mapping demonstrated that the gene order is rpoBC-hupA-rrnE-metA. The rpoBC is located approximately 10 kb proximal to the hupA gene, and rrnE about 8 kb distal to it. Thus, the direction of hupA gene transcription is clockwise on the E. coli map [Bachmann B.J., Microbiol. Rev. 47 (1983) 180-230].

PMID:
3069589
DOI:
10.1016/0378-1119(88)90443-x
[Indexed for MEDLINE]

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