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J Microbiol Immunol Infect. 2019 Jan 10. pii: S1684-1182(18)30490-0. doi: 10.1016/j.jmii.2019.01.001. [Epub ahead of print]

Distribution of virulence genes in bacteremic methicillin-resistant Staphylococcus aureus isolates from various sources.

Author information

1
Division of Infectious Diseases, Department of Medicine, Taipei Veterans General Hospital, Taipei 112, Taiwan; School of Medicine, National Yang-Ming University, Taipei 112, Taiwan. Electronic address: fdwang@vghtpe.gov.tw.
2
Division of Infectious Diseases, Department of Medicine, Taipei Veterans General Hospital, Taipei 112, Taiwan; School of Medicine, National Yang-Ming University, Taipei 112, Taiwan.

Abstract

BACKGROUND/PURPOSE:

Methicillin-resistant Staphylococcus aureus (MRSA) can encode proteins which directly bind bacteria to many tissues and medical devices or catheters to trigger pathogenesis. However, the relationship between genetic backgrounds and virulent factors in MRSA isolates remained incompletely understood yet.

METHODS:

MRSA isolates were collected from blood cultures of patients with infective endocarditis, bone/joint infection, skin/soft tissue infection, or catheter-related bacteremia in hemodialysis at a tertiary medical center between 2005 and 2011. MRSA isolates were characterized by the methods of spa, multilocus sequence, and staphylococcal cassette chromosome mec (SCCmec) typing. Identification of virulence gene expression was measured by Power SYBR Green PCR Master Mix.

RESULTS:

Overall collected were 136 MRSA bacteremic isolates, including those from the cases of infective endocarditis (n = 23), bone/joint infection (n = 49), skin/soft tissue infection (n = 20), or catheter-related bacteremia in patients with acute kidney injury or end-stage renal stage receiving hemodialysis (n = 54). CC8-ST239-MRSA-SCCmec type III-spa type t037 was the most prevalent type observed in all of 136 MRSA bacteremic isolates. The prevalent genes in the group of infective endocarditis were clfA, clfB, fnbA, ebpS, eap, emp, sae, and eno; bone/joint infections clfA, emp, sae, and eno; skin/soft tissue infection eno; hemodialysis catheter-related bacteremia clfA and sae. The distribution of each gene was not statically different among four groups.

CONCLUSIONS:

A major MRSA lineage, CC8-ST239-MRSA-SCCmec type III-spa type t037, is noted among bacteremic MRSA isolates. No disease-specific virulent genes can be identified.

KEYWORDS:

Bacteremia; Staphylococcus aureus; Virulence factors

PMID:
30686615
DOI:
10.1016/j.jmii.2019.01.001
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