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J Mech Behav Biomed Mater. 2019 Apr;92:128-136. doi: 10.1016/j.jmbbm.2019.01.008. Epub 2019 Jan 11.

Shape recovery strain and nanostructures on recovered polyurethane films and their regulation to osteoblasts morphology.

Author information

1
Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Lab for Smart & Bioinspired Materials, College of Bioengineering, Chongqing University, Chongqing 400045, China; Department of Pathophysiology, School of Basic Medical Science, Southwest Medical University, Luzhou, Sichuan 646000, China.
2
Department of Medicinal Chemistry, College of Pharmacy, Southwest Medical University, Luzhou, Sichuan 646000, China.
3
Department of Chemical and Biochemical Engineering, The University of Western Ontario, London, Ontario, Canada N6A 3K7.
4
Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Lab for Smart & Bioinspired Materials, College of Bioengineering, Chongqing University, Chongqing 400045, China.
5
Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Lab for Smart & Bioinspired Materials, College of Bioengineering, Chongqing University, Chongqing 400045, China. Electronic address: yfluo@cqu.edu.cn.

Abstract

Shape memory polyurethanes (SMPUs) have emerged as novel dynamic substrates to regulate cell alignment, in which recovery-induced change in substrates topography has been described as the major contributor. This work, for the first time, confirmed the pivotal roles of recovery strain and phase-separated nanostructures of SMPUs in regulating cell morphology. SMPU films with different stretching ratios (0%, 50%, 100%, and 200%) were found to produce an average recovery strain from 19.41% to 34.04% within 2 h in dulbecco's modified eagle medium (DMEM). Meanwhile, the assembly of hard domains was enhanced during shape recovery, leading to the reorientation of fibrillar apophyses (i.e., nanostructures). Further observation of osteoblast morphology revealed that recovery strain resulted in perpendicular orientation of osteoblasts to strain direction. With the extension of incubation time (24 h), however, the perpendicular orientation was transformed to follow the nanostructures on recovered films, suggesting that the nanostructures might become the determinant of the long-term cell orientation. This study provides a biomechanics-based perspective to understand the dynamic interactions between SMPU and cells, which can help to guide the design of SMPU for specific biomedical applications.

KEYWORDS:

Morphology; Nanostructures; Osteoblasts; Polyurethane; Recovery strain

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