Format

Send to

Choose Destination
Biosens Bioelectron. 2019 Mar 15;129:87-92. doi: 10.1016/j.bios.2018.12.056. Epub 2019 Jan 18.

Optical fiber amplifier for quantitative and sensitive point-of-care testing of myoglobin and miRNA-141.

Author information

1
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China.
2
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China. Electronic address: wwqq99@hnu.edu.cn.
3
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China. Electronic address: kmwang@hnu.edu.cn.

Abstract

A simple, sensitive, quantitative point-of-care testing (POCT) was developed integrating enzyme-linked DNA supersandwich amplification with optical fiber amplifier. The point-of-care (POC) assay can work in both "Turn-off" mode and "Turn-on" mode. Using myoglobin (Myo) and miRNA-141 as the model biomarker, as low as 0.5 nM Myo and 10 pM miRNA-141 could be detected. More importantly, the optical fiber amplifier, which was used as signal readout, exhibited low-cost, small size, rapid response, and easy operation. Although it did not need large equipment and professional technical personnel, the assay was successfully applied to detect Myo and miRNA-141 in 40% human serum. Since the entire process of the strategy could be done directly in the centrifugal tube, requiring a few easy steps, it showed great potential application for early diagnosis in the world, especially in developing countries or remote regions. This work provided a new avenue for developing the portable and sensitive biosensor.

KEYWORDS:

MiRNA-141; Myoglobin; Optical fiber amplifier; Point-of-care testing

PMID:
30685706
DOI:
10.1016/j.bios.2018.12.056
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center