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Free Radic Biol Med. 2019 Apr;134:304-310. doi: 10.1016/j.freeradbiomed.2019.01.027. Epub 2019 Jan 24.

Hydroxytyrosol influences exercise-induced mitochondrial respiratory complex assembly into supercomplexes in rats.

Author information

1
Institute of Nutrition and Food Technology, Biomedical Research Centre, Department of Physiology, Faculty of Sport Sciences, University of Granada, Avda del conocimiento s/n. 18016 Armilla, Granada, Spain.
2
Institute of Biotechnology, Biomedical Research Centre, Department of Physiology, Faculty of Medicine, University of Granada, Avda del conocimiento s/n. 18016 Armilla, Granada, Spain.
3
Institute of Biotechnology, Biomedical Research Centre, Department of Physiology, Faculty of Medicine, University of Granada, Avda del conocimiento s/n. 18016 Armilla, Granada, Spain; Centro de Investigación Biomédica en Red de Fragilidad y Envejecimiento Saludable (CIBERFES), Spain.
4
Institute of Nutrition and Food Technology, Biomedical Research Centre, Department of Physiology, Faculty of Sport Sciences, University of Granada, Avda del conocimiento s/n. 18016 Armilla, Granada, Spain; Department of Biochemistry and Molecular Biology II, School of Pharmacy, University of Granada, Spain.
5
Institute of Nutrition and Food Technology, Biomedical Research Centre, Department of Physiology, Faculty of Sport Sciences, University of Granada, Avda del conocimiento s/n. 18016 Armilla, Granada, Spain. Electronic address: jhuertas@ugr.es.

Abstract

Hydroxytyrosol (HT) has been demonstrated to improve mitochondrial function, both in sedentary and in exercised animals. Herein, we assessed the effects of two different doses of HT on exercise-induced mitochondrial respiratory complex (C) assembly into supercomplexes (SCs) and the relation of the potential results to OPA1 levels and oxidative stress. Wistar rats were allocated into six groups: sedentary (SED), sedentary consuming 20 mg/kg/d of HT (SED-20), sedentary consuming 300 mg/kg/d of HT (SED-300); exercised (EXE), exercised consuming 20 mg/kg/d of HT (EXE-20) and exercised consuming 300 mg/kg/d of HT (EXE-300). Animals were exercised and/or supplemented for 10 weeks, and assembly of SCs, mitochondrial oxidative status and expression of OPA1 were quantified in the gastrocnemius muscle. Both EXE and EXE-20 animals exhibited increased assembly of CI into SCs, but this effect was absent in EXE-300 animals. Levels of CIII2 assembled into SCs were only increased in EXE-20 animals. Notably EXE-300 animals showed a decreased relative expression of s-OPA1 isoforms. Therefore, HT exerted dose-dependent effects on SC assembly in exercised animals. Although the mechanisms leading to SCs assembly in response to exercise and HT are unclear, it seems that a high HT dose can prevent SCs assembly during exercise by decreasing the expression of the s-OPA1 isoforms.

KEYWORDS:

Exercise; Mitochondria; Oxidative phosphorylation; Polyphenols; Skeletal muscle

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