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Bull Environ Contam Toxicol. 2019 Mar;102(3):371-376. doi: 10.1007/s00128-018-2522-2. Epub 2019 Jan 25.

Effect of Chronic Exposure to Methomyl on Tissue Damage and Apoptosis in Testis of Tilapia (Oreochromis niloticus) and Recovery Pattern.

Author information

1
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, No. 9, East Shanshui Road, Binhu District, Wuxi, 214081, Jiangsu, People's Republic of China.
2
Wuxi Fishery College, Nanjing Agricultural University, Wuxi, 214081, People's Republic of China.
3
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, No. 9, East Shanshui Road, Binhu District, Wuxi, 214081, Jiangsu, People's Republic of China. chenjz@ffrc.cn.
4
Wuxi Fishery College, Nanjing Agricultural University, Wuxi, 214081, People's Republic of China. chenjz@ffrc.cn.
5
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, No. 9, East Shanshui Road, Binhu District, Wuxi, 214081, Jiangsu, People's Republic of China. xup@ffrc.cn.
6
Wuxi Fishery College, Nanjing Agricultural University, Wuxi, 214081, People's Republic of China. xup@ffrc.cn.

Abstract

Tilapia were exposed to 0, 0.2, 2, 20, 200 µg/L methomyl for 30 days, and then transferred to methomyl-free water for 18 days. Caspase-8 in serum, apoptosis rate, microstructure and ultra-microstructure of testis were checked after methomyl exposure and at 18 days after transferring to methomyl-free water. There were no significant changes in Caspase-8 activity, apoptosis rate, and tissue structure in testis exposed to 0.2 and 2 µg/L compared with control. However, when tilapia exposed to 20 and 200 µg/L, the Caspase-8 activity and apoptosis rate were induced significantly, and tissue damage happened compared with the control. Thus it would appear 2 µg/L methomyl might be considered as the no observed adverse effect level. Recovery data showed that the effects produced by lower concentration of 20 µg/L were reversible but not at the higher 200 µg/L concentration.

KEYWORDS:

Apoptosis enzyme; Apoptosis rate; Methomyl; Recovery pattern; Tissue structure

PMID:
30683954
DOI:
10.1007/s00128-018-2522-2
[Indexed for MEDLINE]

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