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Environ Pollut. 2019 Feb;245:1086-1094. doi: 10.1016/j.envpol.2018.11.032. Epub 2018 Nov 16.

Examining the responses of the zebrafish (Danio rerio) gastrointestinal system to the suspected obesogen diethylhexyl phthalate.

Author information

1
Department of Environmental and Global Health, University of Florida, Gainesville, FL, 32611, USA; Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL, 32611, USA.
2
Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL, 32611, USA; Department of Physiological Sciences, UF Genetics Institute, University of Florida, Gainesville, FL, 32611, USA.
3
Department of Biostatistics, University of Florida, Gainesville, FL, 32611, USA.
4
Department of Environmental and Global Health, University of Florida, Gainesville, FL, 32611, USA; Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL, 32611, USA; Emerging Pathogens Institute, University of Florida, Gainesville, FL, 32611, USA.
5
Department of Environmental and Global Health, University of Florida, Gainesville, FL, 32611, USA; Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL, 32611, USA. Electronic address: jbisesi@phhp.ufl.edu.

Abstract

Epidemiological evidence suggests that phthalate plasticizers may act as "obesogens", which are chemicals that exacerbate obesity. The gastrointestinal (GI) system is the primary exposure route for phthalates, however, the relationship between phthalate-driven perturbations of GI system functions that can influence obesity has yet to be examined. To address this knowledge gap, we exposed Danio rerio (zebrafish) for 60 days to either (1) Control feeding (5 mg/fish/day), (2) Overfeeding (20 mg/fish/day) or (3) Overfeeding with diethyl-hexyl phthalate (DEHP) (20 mg/fish/day with 3 mg/kg DEHP). After 60 days, Overfed and Overfed + DEHP zebrafish had elevated body mass, and hepatosomatic and gonadosomatic indices. RNAseq analysis of the GI revealed enrichment of gene networks related to lipid metabolism in the Overfed + DEHP group. Many of the enriched networks were under transcriptional control of peroxisome proliferator activated receptor alpha (pparα), a known modulator of lipid metabolism, immune function, and GI function. Real-time PCR confirmed that pparα was overexpressed in the Overfed + DEHP zebrafish, further revealing a pathway by which DEHP may influence lipid metabolism via the GI. These data increase our understanding of phthalate-driven effects on GI function and lipid metabolism, identifying gut-specific gene networks that may drive phthalate-exacerbated obesity.

KEYWORDS:

GI system; Obesity; Peroxisome proliferator activated receptors; Phthalates; RNAseq; Zebrafish

PMID:
30682743
DOI:
10.1016/j.envpol.2018.11.032
[Indexed for MEDLINE]

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