A new bioelectronic nose based on a field effect transistor coupled with an aptamer as the sensing element was developed. The gas-to-liquid extraction interface required for appropriate aptamer function was integrated into standard CMOS technology. It was developed with the use of a sacrificial aluminium etching technique combined with surface modifications by silanes for wettability control. As a proof of concept, aptamer Van74 for vanillin was immobilized on the sensitive surface of the ISFET. The developed microsystem can selectively detect vanillin vapor in a concentration range from 2.7 ppt to 0.3 ppm, with a detection limit of 2.7 ppt. The sensor was able to detect vanillin in a gas sample obtained from roasted coffee beans. This outcome provides a foundation for developing a new generation of bioelectronic noses for the detection and discrimination of volatile compounds.
Keywords: Aptamer; Bioelectronic nose; Gas-to-liquid interface; Hydrophilic surface; Hydrophobic surface; ISFET; Self-assembled monolayers.
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