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Gerontology. 2019;65(2):174-185. doi: 10.1159/000494798. Epub 2019 Jan 24.

Hard Tissue Augmentation of Aged Bone by Means of a Tin-Free PLLA-PCL Co-Polymer Exhibiting in vivo Anergy and Long-Term Structural Stability.

Author information

1
University of Salzburg, Salzburg, Austria.
2
PPPolymer AB, Vällingby, Sweden.
3
University Hospital Vienna, Vienna, Austria.
4
Medical University Innsbruck, Innsbruck, Austriarobert.stigler@i-med.ac.at.

Abstract

BACKGROUND:

Due to aging, tissue regeneration gradually declines. Contemporary strategies to promote tissue-specific regeneration, in particular in elderly patients, often include synthetic material apt for implantation primarily aiming at upholding body functions and regaining appropriate anatomical and functional integrity.

OBJECTIVE:

Biomaterials suitable for complex reconstruction surgical procedures have to exert high physicochemical stability and biocompatibility.

METHOD:

A polymer made of poly-L-lactic acid and poly-ε-caprolactone was synthesized by means of a novel tin-free catalytic process. The material was tested in a bioreactor-assisted perfusion culture and implanted in a sheep model for lateral augmentation of the mandible. Histological and volumetric evaluation was performed 3 and 6 months post-implantation.

RESULTS:

After synthesis the material could be further refined by cryogrinding and sintering, thus yielding differently porous scaffolds that exhibited a firm and stable appearance. In perfusion culture, no disintegration was observed for extended periods of up to 7 weeks, while mesenchymal stromal cells readily attached to the material, steadily proliferated, and deposited extracellular calcium. The material was tested in vivo together with autologous bone marrow-derived stromal cells. Up to 6 months post-implantation, the material hardly changed in shape with composition also refraining from foreign body reactions.

CONCLUSION:

Given the long-term shape stability in vivo, featuring imperceptible degradation and little scarring as well as exerting good compatibility to cells and surrounding tissues, this novel biomaterial is suitable as a space filler in large anatomical defects.

KEYWORDS:

3D culture; Hard tissue; Mesenchymal stem cells; Regeneration

PMID:
30677770
DOI:
10.1159/000494798

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