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Nanomedicine (Lond). 2019 Feb;14(3):317-333. doi: 10.2217/nnm-2018-0161. Epub 2019 Jan 24.

Antibody-functionalized gold nanoparticles as tumor-targeting radiosensitizers for proton therapy.

Author information

1
Research Center for the Physics of Matter & Radiation (PMR-LARN), Namur Research Institute for Life Sciences (NARILIS), University of Namur, B-5000 Namur, Belgium.
2
Unité de Recherche en Biologie Cellulaire (URBC), Namur Research Institute for Life Sciences (NARILIS), University of Namur, B-5000 Namur, Belgium.
3
Biomedical Magnetic Resonance Group (REMA), Louvain Drug Research Institute, Université Catholique de Louvain, B-1200 Woluwé, Saint Lambert, Belgium.
4
Pole of Pharmacology & Therapeutics (FATH), Institut de Recherche Expérimentale et Clinique (IREC), UCL (Université Catholique de Louvain), B-1200 Brussels, Belgium.
5
Department of Radiotherapy & Oncology, CHU & University of Liège, B-4000 Liège, Belgium.

Abstract

AIM:

This study aimed at developing antibody-functionalized gold nanoparticles (AuNPs) to selectively target cancer cells and probing their potential radiosensitizing effects under proton irradiation.

MATERIALS & METHODS:

AuNPs were conjugated with cetuximab (Ctxb-AuNPs). Ctxb-AuNP uptake was evaluated by transmission electron microscopy and atomic absorption spectroscopy. Radioenhancing effect was assessed using conventional clonogenic assay.

RESULTS & CONCLUSION:

Ctxb-AuNPs specifically bound to and accumulated in EGFR-overexpressing A431 cells, compared with EGFR-negative MDA-MB-453 cells. Ctxb-AuNPs enhanced the effect of proton irradiation in A431 cells but not in MDA-MB-453 cells. These data indicate, for the first time, that combining enhanced uptake by specific targeting and radioenhancing effect, using conjugated AuNPs, is a promising strategy to increase cell killing by protontherapy.

KEYWORDS:

EGFR; gold nanoparticles; nanoparticle uptake; protontherapy; radiosentizing effects

PMID:
30675822
DOI:
10.2217/nnm-2018-0161
[Indexed for MEDLINE]

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