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Cell Biosci. 2019 Jan 17;9:11. doi: 10.1186/s13578-019-0273-3. eCollection 2019.

Long non-coding RNA ANRIL-mediated inflammation response is involved in protective effect of rhein in uric acid nephropathy rats.

Author information

1
1Department of Traditional Chinese Medicine, Renmin Hospital of Wuhan University, No. 99 Zhangzhidong Road, Wuhan, 430060 China.
2
2Department of Acupuncture and Moxibustion, Renmin Hospital of Wuhan University, Wuhan, 430060 China.

Abstract

Background:

The aim of this study was to investigate the role of long non-coding RNAs (LncRNAs) antisense non-coding RNA in the INK4 locus (ANRIL) in anti-inflammation of rhein in uric acid nephropathy (UAN) rats.

Methods:

Rat models of UAN were induced by adenine and potassium oxonate. Enzyme-linked immunosorbent assay (ELISA) was performed to assess inflammation factor in serum and supernatant. ANRIL mRNA level was detected using real-time reverse transcription PCR (qRT-PCR). Immunostaining was used to observe pathological changes of renal tissues in rats.

Results:

ANRIL and inflammatory factor levels were highly expressed in patient with UAN. Furthermore, rhein showed an observable effect on anti-inflammatory and renal protection in UAN rats, rhein inhibited expressions of ANRIL in vivo or in vitro. Besides, ANRIL-mediated inflammatory response attenuated protective effect of rhein.

Conclusions:

ANRIL-mediated inflammatory response attenuated the protective effect of rhein in UAN rats. This study showed an understanding of the role and mechanism of ANRIL in UAN, which provides a new target and therapy for the prevention and treatment of UAN.

KEYWORDS:

Hyperuricemia; Inflammatory response; Long non-coding RNA ANRIL; Rhein; UAN

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