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Acta Diabetol. 2019 Feb;56(2):227-236. doi: 10.1007/s00592-019-01286-w. Epub 2019 Jan 23.

Duodenal adipose tissue is associated with obesity in baboons (Papio sp): a novel site of ectopic fat deposition in non-human primates.

Author information

1
Department of Genetics, Texas Biomedical Research Institute, PO Box 760549, San Antonio, TX, 78245-0549, USA. paulb.higgins@gmail.com.
2
Endocrinology and Metabolism, Department of Health Science, University of Milan, Via A. di Rudini, 8, 20142, Milan, Italy. franco.folli@unimi.it.
3
UOSD of Diabetes and Metabolic Disorders, ASST Santi Paolo e Carlo, Milan, Italy. franco.folli@unimi.it.
4
Center for Laboratory Animal Breeding, Oswaldo Cruz Foundation, Oswaldo Cruz, Rio de Janeiro, Brazil.
5
Department of Genetics, Texas Biomedical Research Institute, PO Box 760549, San Antonio, TX, 78245-0549, USA.
6
Laboratory of Clinical Biochemistry and Mass Spectrometry, Department of Health Science, University of Milan, Milan, Italy.
7
Department of Obstetrics and Gynecology, School of Medicine, Texas Tech University Health Sciences Center at the Permian Basin, Odessa, TX, USA.
8
Nutrition Research Institute, Department of Nutrition, University of North Carolina at Chapel Hill, Kannapolis, NC, USA.
9
Southwest National Primate Research Center, Texas Biomedical Research Institute, San Antonio, TX, USA.
10
Metabolic Research Laboratory, University of Guanajuato, Guanajuato, Mexico.
11
Pathological Anatomy, Department of Health Science, University of Milano, Via A. di Rudini' 8, 20142, Milan, Italy.
12
ASST Santi Paolo e Carlo, Milan, Italy.
13
Department of Biomedical and Clinical Sciences, "L. Sacco", University of Milan, Milan, Italy.
14
Department of Health Science, University of Milan, Milan, Italy.
15
The Obesity Society, 1110 Bonifant St. Silver Spring, Maryland, USA.

Abstract

AIMS:

Ectopic fat is a recognized contributor to insulin resistance and metabolic dysfunction, while the role of fat deposition inside intestinal wall tissue remains understudied. We undertook this study to directly quantify and localize intramural fat deposition in duodenal tissue and determine its association with adiposity.

METHODS:

Duodenal tissues were collected from aged (21.2 ± 1.3 years, 19.5 ± 3.1 kg, n = 39) female baboons (Papio sp.). Fasted blood was collected for metabolic profiling and abdominal circumference (AC) measurements were taken. Primary tissue samples were collected at the major duodenal papilla at necropsy: one full cross section was processed for hematoxylin and eosin staining and evaluated; a second full cross section was processed for direct chemical lipid analysis on which percentage duodenal fat content was calculated.

RESULTS:

Duodenal fat content obtained by direct tissue quantification showed considerable variability (11.95 ± 6.93%) and was correlated with AC (r = 0.60, p < 0.001), weight (r = 0.38, p = 0.02), leptin (r = 0.63, p < 0.001), adiponectin (r = - 0.32, p < 0.05), and triglyceride (r = 0.41, p = 0.01). The relationship between duodenal fat content and leptin remained after adjusting for body weight and abdominal circumference. Intramural adipocytes were found in duodenal sections from all animals and were localized to the submucosa. Consistent with the variation in tissue fat content, the submucosal adipocytes were non-uniformly distributed in clusters of varying size. Duodenal adipocytes were larger in obese vs. lean animals (106.9 vs. 66.7 µm2, p = 0.02).

CONCLUSIONS:

Fat accumulation inside the duodenal wall is strongly associated with adiposity and adiposity related circulating biomarkers in baboons. Duodenal tissue fat represents a novel and potentially metabolically active site of ectopic fat deposition.

KEYWORDS:

Adipose tissue; Baboons; Duodenum; Ectopic fat deposition; Gastrointestinal tract; Insuline resistance; Non human primates

PMID:
30673859
PMCID:
PMC6691506
[Available on 2020-02-01]
DOI:
10.1007/s00592-019-01286-w
[Indexed for MEDLINE]
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