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RNA. 2019 Apr;25(4):465-471. doi: 10.1261/rna.069799.118. Epub 2019 Jan 22.

Assembly and early maturation of large subunit precursors.

Author information

1
Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, New York 10065, USA.
2
Tri-Institutional Training Program in Chemical Biology, The Rockefeller University, New York, New York 10065, USA.

Abstract

The eukaryotic ribosome is assembled through a complex process involving more than 200 factors. As preribosomal RNA is transcribed, assembly factors bind the nascent pre-rRNA and guide its correct folding, modification, and cleavage. While these early events in the assembly of the small ribosomal subunit have been relatively well characterized, assembly of the large subunit precursors, or pre-60S, is less well understood. Recent structures of nucleolar intermediates of large subunit assembly have shed light on the role of many early large subunit assembly factors, but how these particles emerge is still unknown. Here, we use the expression and purification of truncated pre-rRNAs to examine the initial assembly of pre-60S particles. Using this approach, we can recapitulate the early recruitment of large subunit assembly factors mainly to the domains I, II, and VI of the assembling 25S rRNA.

KEYWORDS:

RNA; biochemistry; ribosome biogenesis

PMID:
30670483
PMCID:
PMC6426289
[Available on 2020-04-01]
DOI:
10.1261/rna.069799.118
[Indexed for MEDLINE]

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