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Phytomedicine. 2018 Sep 27;57:9-17. doi: 10.1016/j.phymed.2018.09.034. [Epub ahead of print]

Inhibition of neutrophil migration and reduction of oxidative stress by ethyl p-coumarate in acute and chronic inflammatory models.

Author information

1
Medicinal Plants Research Center, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, 64049-550 Teresina, Piauí, Brazil.
2
Laboratory of Experimental Physiopharmacology, Federal University of Piauí, Parnaíba, Piauí, Brazil.
3
Medicinal Plants Research Center, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, 64049-550 Teresina, Piauí, Brazil. Electronic address: carlos_mlf@hotmail.com.
4
Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Ceará, Brazil.
5
Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Ceará, Brazil. Electronic address: robertocesar@ufc.br.
6
Laboratory of Experimental Physiopharmacology, Federal University of Piauí, Parnaíba, Piauí, Brazil. Electronic address: jandvenes@ufpi.edu.br.
7
Department of Pharmaceutical Sciences, Federal University of Paraíba, João Pessoa, Paraíba, Brazil.
8
Medicinal Plants Research Center, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, 64049-550 Teresina, Piauí, Brazil. Electronic address: fassisol@ufpi.edu.br.

Abstract

BACKGROUND:

It is well known that medicinal plants and their products are relevant candidates for the treatment of inflammatory conditions. Ethyl p-coumarate is a phenylpropanoid that has similar structure to others anti-inflammatory and antioxidant substances. However, these activities have never been tested.

PURPOSE:

The aim of this study was to investigate the effect of ethyl p-coumarate on inflammatory and oxidative stress parameters.

STUDY DESIGN:

This is an experimental study to evaluate the anti-inflammatory and antioxidant activities of ethyl p-coumarate in acute and chronic models of inflammation.

METHODS:

The anti-inflammatory effect of ethyl p-coumarate was evaluated in Swiss mice by carrageenan-induced paw edema model (1%, 50 μl), followed by histological analysis, and edema induced by compound 48/80 (12 µg/paw), histamine (100  µg/paw), serotonin (100 µg/paw) and prostaglandin E2 (3 nmol/paw) in comparison to indomethacin treatment (10 mg/kg, p.o.). In addition, peritonitis was induced by carrageenan (500 μg/cavity) to neutrophil and total leukocytes counting, myeloperoxidase (MPO), interleukin 6 (IL-6) and 8 (IL-8), nitrite (NO2-), glutathione (GSH) and malondialdehyde (MDA) measurements. The arthritis model was induced with Freund's complete adjuvant (id. 0.1 ml) in female Wistar rats, with measurement of joint diameter and X-ray. Changes in gastric tissue of Swiss mice were analyzed in comparison to indomethacin (20  mg/kg, p.o.).

RESULTS:

After treatment with ethyl p-coumarate, the animals had no apparent toxic effects, and significantly inhibited paw edema induced by edematogenic agents, neutrophil (p < 0.001) and total leukocyte (p < 0.001) migration, MPO (p < 0.01), IL-6 (p < 0.05) and IL-8 (p < 0.5), MDA (p < 0.5), GSH (p < 0.5), NO2- (p < 0.001), joint thickness and bones changes. Furthermore, were not observed significant formation of gastric lesions.

CONCLUSION:

Taken together, these results suggest that ethyl p-coumarate exhibits anti-inflammatory activity through the inhibition of inflammatory mediators and leukocyte migration without causing gastric lesions.

KEYWORDS:

Arthritis; Cytokines; Inflammation; Neutrophils; Phenylpropanoid

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