Format

Send to

Choose Destination
Methods Mol Biol. 2019;1913:33-48. doi: 10.1007/978-1-4939-8979-9_3.

Systems-Level Immune Monitoring by Mass Cytometry.

Author information

1
Science for Life Laboratory, Division of Clinical Paediatrics, Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden.
2
Science for Life Laboratory, Division of Clinical Paediatrics, Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden. petter.brodin@ki.se.
3
Department of Newborn Medicine, Karolinska University Hospital, Stockholm, Sweden. petter.brodin@ki.se.

Abstract

As therapies involving the modulation, stimulation, and deliberate excitation of the immune system are becoming routine, better methods for monitoring immune responses in human patients are needed. Mass cytometry allows for detailed profiling of all immune cell populations and their functional responses using a simple blood sample. When combined with appropriate computational analyses, the resolution for distinguishing desired responses from unproductive or even adverse reactions to immunotherapeutic interventions increases. Here we describe a core experimental and computational framework for global, systems-level immune monitoring by mass cytometry.

KEYWORDS:

CyTOF; Human immunology; Immune monitoring; Mass cytometry; Systems immunology; Tumor immunology

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center