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Nat Methods. 2019 Feb;16(2):171-174. doi: 10.1038/s41592-018-0294-6. Epub 2019 Jan 21.

A genetically encoded near-infrared fluorescent calcium ion indicator.

Author information

1
Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
2
Media Lab and McGovern Institute for Brain Research, MIT, Cambridge, MA, USA.
3
Institute for Biological and Medical Imaging, Helmholtz Center Munich, Neuherberg, Germany.
4
Faculty of Medicine, Technical University of Munich, Munich, Germany.
5
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
6
Department of Pharmacology, University of California San Diego, La Jolla, CA, USA.
7
Department of Cell Biology and Neuroscience, Montana State University, Bozeman, MT, USA.
8
Departments of Ophthalmology and of Neuroscience and Physiology, New York University Langone Health, New York City, NY, USA.
9
Faculty of Medicine and Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland.
10
Department of Information Technology and Electrical Engineering and Institute for Biomedical Engineering, ETH Zurich, Zurich, Switzerland.
11
Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada. robert.e.campbell@ualberta.ca.
12
Department of Chemistry, Graduate School of Science, The University of Tokyo, Tokyo, Japan. robert.e.campbell@ualberta.ca.

Abstract

We report an intensiometric, near-infrared fluorescent, genetically encoded calcium ion (Ca2+) indicator (GECI) with excitation and emission maxima at 678 and 704 nm, respectively. This GECI, designated NIR-GECO1, enables imaging of Ca2+ transients in cultured mammalian cells and brain tissue with sensitivity comparable to that of currently available visible-wavelength GECIs. We demonstrate that NIR-GECO1 opens up new vistas for multicolor Ca2+ imaging in combination with other optogenetic indicators and actuators.

PMID:
30664778
PMCID:
PMC6393164
DOI:
10.1038/s41592-018-0294-6
[Indexed for MEDLINE]
Free PMC Article

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