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RNA Biol. 2019 Mar;16(3):282-294. doi: 10.1080/15476286.2019.1572435. Epub 2019 Jan 29.

miR-151-5p modulates APH1a expression to participate in contextual fear memory formation.

Author information

1
a Institute of Brain Science and Disease, School of Basic Medicine , Qingdao University , Qingdao , Shandong , People's Republic of China.
2
b The Royal, Department of Psychiatry, and Department of Cellular and Molecular Medicine , University of Ottawa Institute of Mental Health Research , Ottawa , Canada.
3
c Department of Cell and Neurobiology , School of Basic Medicine, Shandong University , Jinan , Shandong , People's Republic of China.
4
d BGI-Shenzhen , Shenzhen , People's Republic of China.
5
e Department of Breast Surgery , Qilu hospital, Shandong University , Jinan , Shandong , People's Republic of China.

Abstract

Long-term memory formation requires gene expression and new protein synthesis. MicroRNAs (miRNAs), a family of small non-coding RNAs that inhibit target gene mRNA expression, are involved in new memory formation. In this study, elevated miR-151-5p (miR-151) levels were found to be responsible for hippocampal contextual fear memory formation. Using a luciferase reporter assay, we demonstrated that miR-151 targets APH1a, a protein that has been identified as a key factor in γ-secretase activity, namely APH1a. Blocking miR-151 can upregulate APH1a protein levels and subsequently impair hippocampal fear memory formation. These results indicate that miR-151 is involved in hippocampal contextual fear memory by inhibiting APH1a protein expression. This work provides novel evidence for the role of miRNAs in memory formation and demonstrates the implication of APH1a protein in miRNA processing in the adult brain.

KEYWORDS:

APH1a; hippocampus; memory; mir-151-5p

PMID:
30663934
PMCID:
PMC6380341
[Available on 2020-01-29]
DOI:
10.1080/15476286.2019.1572435
[Indexed for MEDLINE]

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