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Hematology. 2019 Dec;24(1):300-307. doi: 10.1080/16078454.2019.1568354.

Differentially expressed plasma proteins of β-thalassemia/hemoglobin E patients in response to curcuminoids/vitamin E antioxidant cocktails.

Author information

1
a Department of Biochemistry, Faculty of Medicine Siriraj Hospital , Mahidol University , Bangkok , Thailand.
2
b Laboratory of Biochemistry , Chulabhorn Research Institute , Bangkok , Thailand.
3
c Department of Molecular Biotechnology and Bioinformatics, Faculty of Science , Prince of Songkla University , Songkla , Thailand.
4
d Applied Biological Sciences Program, Chulabhorn Research Institute , Bangkok , Thailand.

Abstract

OBJECTIVE:

Iron overload and oxidative stress are the major causes of serious complications and mortality in thalassemic patients. Our previous work supports the synergistic effects of antioxidant cocktails (curcuminoids or vitamin E, N-acetylcysteine, and deferiprone) in treatment of β-thalassemia/Hb E patients. This further 2-DE-based proteomic study aimed to identify the plasma proteins that expressed differentially in response to antioxidant cocktails.

METHODS:

Frozen plasma samples of ten normal subjects and ten β-thalassemia/Hb E patients at three-time points (baseline, month 6, and month 12) were reduced the dynamic range of proteome using ProteoMiner kit and separated proteins by two-dimensional gel electrophoresis. Differentially expressed proteins were identified using tandem mass spectrometry. Several plasma proteins were validated by ELISA and Western blot analysis.

RESULTS:

Thirteen and 11 proteins were identified with altered expression levels in the curcuminoids- and vitamin E cocktail groups, respectively. The associations between vitronectin (VTN) expression and total bilirubin levels, as well as between serum paraoxonase/arylesterase 1 (PON1) expression and blood reactive oxygen species were observed. Validation results were consistent with proteomics results.

DISCUSSION AND CONCLUSIONS:

These plasma proteins may provide better understanding of the mechanisms underlying the therapeutic effects of antioxidant cocktails in thalassemic patients.

KEYWORDS:

N-acetylcysteine; antioxidant; curcuminoids; deferiprone; plasma proteomics; thalassemia; vitamin E; β-thalassemia/Hb E

PMID:
30661467
DOI:
10.1080/16078454.2019.1568354
[Indexed for MEDLINE]

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